摘要
目的:研究红景天醇提物对小鼠宫颈癌U14生长的抑制作用及对增殖细胞核抗原(PCNA)和突变型p53蛋白表达的影响.方法:观察红景天醇提物对U14实体瘤和腹水瘤的抑瘤率和生命延长率,并用免疫组化SP法检测PCNA和突变型p53蛋白阳性细胞表达数.结果:以阴性对照组为基数,红景天醇提物3个剂量组(500 mg.kg-1,1 000mg.kg-1和1 500 mg.kg-1体重)及环磷酰胺(CTX)阳性对照组实体瘤各组抑瘤率分别为39.31%,59.31%,67.59%,73.79%(P<0.01);腹水瘤各组生命延长率分别为45.98%,60.40%,78.93%,83.65%(P<0.01).同时各组药物对PCNA和突变型p53蛋白的表达水平也有不同程度的抑制作用(P<0.01,P<0.05).结论:红景天对小鼠宫颈癌U14的生长有一定的抑制作用,其机制可能与抑制PCNA和突变型p53蛋白的表达有关.
Objective To study the antitumor effect of Rhodiola rosea on mice bearing cervical carcinoma(U14) and the expression of PCNA(proliferating cell nuclear antigen) and p53.Methods The effect of the ethanolic extract of Rhodiola rosea on the inhibition rate of solid tumor and the life span of ascites tumor were calculated by the animal tumor model experiment in vivo.The positive cell numbers of PCNA and mutant p53 protein were measured by immunohistochemical SP method.Results In compare with the control group,t...
出处
《西北民族大学学报（自然科学版）》
2009年第4期39-42,共4页
Journal of Northwest Minzu University(Natural Science)
基金
西北民族大学校中青年基金
