摘要
采用酸性溶胶法在碳纳米管(CNT)上负载纳米二氧化钛(TiO2)颗粒,并制备了CNT-TiO2修饰电极,利用TiO2与辣根过氧化物酶(HRP)间的静电吸附实现了HRP在CNT-TiO2修饰电极表面的固定。利用硫化物对HRP的抑制作用实现了对硫化物的检测。其峰电流值与硫化物的浓度在0.75×10-7～10.5×10-6mol/L范围内呈线性关系。方法的检出限为(3S/N)0.206×10-7mol/L。
The composite material of nano-meter titanium dioxide decorated carbon nanotubes (CNT-TiO2) was prepared by acid sol method.Horseradish peroxidase was successfully immobilized on a CNT-TiO2 modified gold electrode (HRP/CNT-TiO2/Au).Orthoaminophenol was chosen as the electron transfer mediator in the system.A novel inhibition-based HRP biosensor for sulfides detection is described.The response of the sensor is based on inhibitory effect of sulfides on the electrocatalytic current of the HRP sensor.The peak c...
出处
《分析试验室》
CAS
CSCD
北大核心
2010年第6期69-72,共4页
Chinese Journal of Analysis Laboratory
基金
浙江省自然科学基金(Y4080223)
浙江省教育厅基金(20071277)项目资助
