摘要
目的:测定重组变形链球菌GS5葡糖基转移酶GTF(glucosyltransferase)催化活性区的活性。方法:通过异丙基硫代-β-D-半乳糖苷(IPTG)诱导含有质粒pET32-NusA-CAT的大肠杆菌,制备可溶性的重组GTF;利用蒽酮硫酸液测定重组GTF与对照样品活性,酶联仪读取OD630数值。结果:蒽酮硫酸液测定重组GTF,对照样品和蔗糖孵育后所得产物OD630数值有明显统计学差异(P<0.05)。结论:重组GTF的CAT区具有催化蔗糖生成葡聚糖的生物学活性。
Objective: To assay the activity of recombinant expressed the catalytic region of gtfC from S. mutans GS5. Method: Obtain the soluble fusion protein by IPTG induce the E.coli BL21, Anthrone-sulphuric acid test the activity of the soluble recombinant protein (rGTF). Results: OD630 fig show there are apparently difference between the rGTF group and the blank group (P < 0.05) by the mothod of anthrone-sulphuric acid. Conclusions: The results showed that the soluble rGTF could catalyze the sucrose effectivel...
出处
《口腔颌面修复学杂志》
2010年第2期65-67,共3页
Chinese Journal of Prosthodontics
基金
国家自然科学基金资助项目(项目编号:30440059)
关键词
变形链球菌
葡糖基转移酶
蒽酮硫酸液
dental caries
glucosyltransferases
anthrone-sulphuric acid
