摘要
目的探讨参芪扶正注射液对犬体外循环(CPB)缺血再灌注(I/R)肺细胞凋亡的影响及其可能机制。方法建立犬CPB模型,将实验犬随机分为CPB I/R组(对照组,n=6)和参芪扶正注射液治疗组(治疗组,n=6),在CPB前10 min(T1),主动脉开放后60 min(T2)、120 min(T3)在各相应时段采集同一肺叶肺组织1.0 cm3在同一固定液中固定,常规石蜡包埋切片,切片厚度为5μm,用SP免疫组织化学法检测Caspase-3、Bcl-2、Bax蛋白表达。结果在再灌注后60 min、120 min治疗组Caspase-3、Bax表达均较对照组减弱(P<0.05),Bcl-2表达则较对照组增强(P<0.05),Bcl-2/Bax较对照组增强(P<0.01)。结论在犬CPB过程中应用参芪扶正注射液,可以降低Caspase-3、Bax蛋白的表达,提高Bcl-2的表达,从而抑制肺泡细胞凋亡。
Objective To study the effect and possible mechanism of SenQi FuZheng injection on lung cell apoptosis in dogs with ischemia reperfusion(I/R) following cardiopulmonary bypass(CPB).Methods CPB dog models were established.The 12 mongrel dogs were randomly divided into two groups: CPB I/R group(control) and SenQi FuZheng injection group(therapy).The lung tissue samples of 1.0 cm3 from the same lobes of lung were obtained at 10 min before CPB(T1) and 60(T2),120 min(T3) after reperfusion.These samples were fixed and then embedded in paraffin imbedding,and cut into thin slices(5μm).Streptavidin/peroxidase(SP) immunohistochemical method was employed to detect Caspase-3,Bcl-2,and Bax protein expressions.Results The expressions of Caspase-3 and Bax protein in the tissues with SenQi FuZheng therapy were significantly lower at 60 and 120 min after reperfusion than the controls(P<0.05).The expression of Bcl-2 protein and the Bcl-2/Bax ratio in the tissues with SenQi FuZheng therapy were significantly higher at 60 and 120 min after reperfusion than the controls(P<0.05).Conclusion Administration of SenQi FuZheng injection in CPB dogs can inhibit apoptosis of alveolar cells by reducing the expressions of Caspase-3 and Bax protein and increasing the expression of Bcl-2 protein.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2010年第4期613-616,共4页
Journal of Sichuan University(Medical Sciences)
基金
遵义市科技基金[遵义市科合社字(2007)01号]资助
关键词
参芪扶正注射液
体外循环
缺血再灌注
细胞凋亡
犬
SenQi FuZheng injection Cardiopulmonary bypass Ischemia reperfusion Cell apoptosis Dog
