摘要
采集疑似猪肺炎支原体病理变化的肺脏,接种到改良牛心培养基、猪胃消化液培养基进行培养,经瑞特染色、镜检,菌落狄氏染色、PCR鉴定及序列比对、生化试验鉴定,抗猪肺炎支原体血清生长抑制试验,分离获得一株猪肺炎支原体云南地方流行菌株,命名为YN200901。试验结果为云南本地株猪肺炎支原体的后续研究提供了物质基础。
Samples from swine lungs with suspected pathological lesions due to swine enzootic pneumonia were collected,inoculated into improved cattle heart and pig stomach digestion medium for isolation and culture.Wright’s staining,bacterial colony of disse staining,bacterial L-type identification,biochemical tests,anti-Mycoplasma hyopneumoniae growth inhibition test as well as PCR and sequence alignments for comfirming the bacteria strain were conducted.Based on the results,strain was isolated as swine pneumonia mycoplasma,enzootic in Yunnan province named YN200901 which can be applicable studies.
出处
《动物医学进展》
CSCD
北大核心
2010年第9期22-25,共4页
Progress In Veterinary Medicine
基金
云南省高端科技人才引进计划项目(2009CI125)
关键词
猪肺炎支原体
YN200901株
分离鉴定
Mycoplasma hyopneumoniae
YN200901 strain
isolation and identification
