摘要
建立了一种检测方便面中丙烯酰胺的液相色谱方法。萃取剂选择1g/L的蚁酸溶液,将萃取液以12000r/min,2℃离心18min,上清液再以14500r/min,0℃离心18min,可分离基质中淀粉、蛋白质、脂肪等干扰物;方法采用ZORBAX SB-C18(250mm×4.6mm,5μm)色谱柱,流动相为A(乙腈∶水=1∶24(V/V))和B(乙腈),流速0.5mL/min,在丙烯酰胺的最大吸收197nm处检测,提高了方法的检出限及回收率。该方法有良好的线性关系(r=1.00000),检出限为48.0μg/kg。回收率94.4%～109.8%,相对标准偏差为4.9%～5.6%。
A fast and precise method for the determination of acrylamide in fried instant noodles with high performance liquid chromatography (HPLC) was established.The method is based on extraction of acrylamide with 1 g/L HCOOH solution.The aqueous sample solution was centrifuged twice at 12,000 rpm,2 ℃ and 14,500 rpm,0 ℃ for 18 min to successfully remove the amylum,protein and fat in the samples.The method was optimized for the determination of acrylamide in fried instant noodle samples as follows:separation is car...
出处
《分析试验室》
CAS
CSCD
北大核心
2010年第11期41-44,共4页
Chinese Journal of Analysis Laboratory
基金
国家质检总局科技计划:(2009IK199)
新疆大学校基金(2007310302)项目资助
关键词
高效液相色谱
丙烯酰胺
方便面
High performance liquid chromatography
Acrylamide
Fried instant noodle
