摘要
目的通过动态观察亚甲蓝、纳米活性炭及中华墨汁在大鼠胃癌淋巴结染色中的示踪作用,比较几种常见显像剂在胃癌中的显像效率,为进一步的临床应用和研究提供实验依据。方法应用Walker-256细胞株建立鼠种植性胃癌模型,将36只胃肿瘤大鼠随机分为3组,分别为亚甲蓝组、纳米活性炭组及中华墨汁组。观察各组淋巴结染色时间、染色枚数、褪色时间、肝肾功能等指标。结果注射后,染料到达大鼠胃癌第1站的淋巴结显像时间分别为中华墨汁组(20.00±8.53)min,纳米活性炭组(4.11±1.59)min,亚甲蓝组(14.33±2.22)s。染料运行至第2站淋巴结的时间,亚甲蓝组为(21.88±2.38)s,纳米活性炭组为(13.75±4.61)min,中华墨汁组为>1h。经统计分析可知,不同染料间在到达1及第2站淋巴结时间上差异有统计学意义(P=0.00),三者在显像时间上差异明显,亚甲蓝较活性炭显像快,中华墨汁明显长于前两者。染色枚数亚甲蓝组为(7.83±1.75)枚,活性炭组为(7.25±1.77)枚,两者比较无明显差异(P=0.352),中华墨汁组为(2.17±0.84)枚,与前两者均有显著差异(P=0.000)。在褪色时间上,亚甲蓝在(102.08±10.74)min完全褪色,活性炭>2h,中华墨汁>24h,三者差异显著。结论亚甲蓝、纳米活性炭可以作为胃癌淋巴结的染色示踪剂,显像效率相仿,但两者各具优缺点,中华墨汁组显像效率较前两者低,与其性状及浓度有关。
Objective To observe the dyeing function and visualization efficiency of methylene blue(MB),nanometric activated carbon and chinese ink in the lymphatic mapping of mice gastric xenograft tumor primarily.Methods Walker-256 gastric cancer cells were inoculated into stomach wall in 36 mice successfully.Then,36 mice with xenograft tumor were divided randomly into 3 equal groups (12 mice per group),namely MB group,Nanometric Activated Carbon group and Chinese Ink group.Finally,we observed the dying time of the lymph nodes,the number of the dying nodes,and the fading time of the dying lymph.Results The time of the first station lymph node staining was 14.33±2.22 s,13.75±4.61 min,20.00±8.53 min using MB,Nanometric Activated Carbon,and Chinese Ink,respectively.The time of the second station lymph node staining was 21.88±2.38 s,13.75±4.61 min,>1 h.there was significant difference between MB group and Nanometric Activated Carbon group or Chinese Ink group(P24 h,in MB group,Nanometric Activated Carbon group and Chinese Ink group,respectively and there was significant difference between MB group and Nanometric Activated Carbon group or Chinese Ink group (P<.01).Conclusion Methylene Blue and Nanometric Activated Carbon may be used as high efficient labelled compound for Lymph Nodes in gastric tumor,but there is no obvious difference with respect to the visualization efficiency.However,Chinese Ink was not a good labelled compound comparing to Methylene Blue and Nanometric Activated Carbon.
出处
《实用癌症杂志》
2010年第5期453-456,共4页
The Practical Journal of Cancer
关键词
胃癌
亚甲蓝
纳米活性炭
中华墨汁
示踪剂法
Stomach tumor
Methylene blue
Nanometric activated carbon
Chinese ink
Labelled compound
