摘要
BMPR-IB基因是发现最早且对绵羊排卵率影响机理已经阐明的多胎主效基因,主要在与繁殖有关的组织和器官中表达,并且对绵羊排卵起重要的调控作用。本研究采用实时荧光定量PCR(Real-time Quantitative PCR,RQ-PCR)技术的相对定量方法对BMPR-IB基因在不同生理时期蒙古羊的卵巢和子宫组织进行了差异表达的研究。定量结果得到-βActin基因的扩增曲线回归方程为y=-3.358x+35.708,回归系数R2=0.990,BMPR-IB基因的扩增曲线回归方程为y=-2.119x+31.424,回归系数R2=0.992。以非发情期蒙古羊的卵巢组织定量结果为对照计算得到BMPR-IB基因在发情期蒙古羊的卵巢组织中表达量最高,在发情期的子宫组织中表达量最低,在发情期卵巢组织中的表达量是非发情期的2.65倍。
The BMPR-IB gene is the firstly discovered gene which the mechanism on ovulation rate of ovine has been clarified.BMPR-IB gene was mainly expressed in reproductive tissues and organs and played an important role in regulation of ovulation in sheep.The current study examined expressional patterns of BMPR-IB gene in the ovarian and uterus tissues at different stages in Mongolian sheep by using relative quantitative Real-time Quantitative PCR(RQ-PCR).The results showed that the regression equation of β-Actin g...
出处
《畜牧与兽医》
北大核心
2010年第8期6-9,共4页
Animal Husbandry & Veterinary Medicine
基金
"内蒙古农牧业科学院青年科技创新基金项目
国家科技攻关计划-优质肉用羊新品种选育与产业化开发(2008BADB2B04-3)
国家现代肉羊产业技术体系建设专项(农科教发[2008]10号)"资助
关键词
蒙古羊
BMPR-IB基因
差异表达
实时荧光定量PCR
Mongolian sheep
Bone Morphogenetic Protein Receptor-IB Gene
Differential Expression
Real-time Quantitative PCR
