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Fas counterattack in cholangiocarcinoma:a mechanism for immune evasion in human hilar cholangiocarcinomas 被引量:6

Fas counterattack in cholangiocarcinoma:a mechanism for immune evasion in human hilar cholangiocarcinomas
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摘要 AIM: To investigate FasL expression in hilar cholangiocarcinoma tissues and cultured cholangiocarcinoma cells, and to assess its ability to induce apoptosis. METHODS: We studied the expression of FasL by human hilar cholangiocaroinomas tissues by immunohistochemistry, and the QBC939 cholangiocarcinoma cell line by RT-PCR, immunohistochemistry, and Western Blot. TUNEL and flow cytometry were used to detect apoptotic cells. RESULTS: Prevalent expression of FasL was detected in 39 resected hilar cholangiocarcinoma tissues. TUNEL staining disclosed a high level of cell death among lymphocytes infiltrating FasL positive areas of tumor. FasL mRNA and protein expressions in cholangiocarcinoma cells could induce Jurkat cells. CONCLUSION: Hilar cholangiocarcinomas may elude immunological surveillance by inducing, via Fas/FasL system, the apoptosis of activated lymphocytes. AIM: To investigate FasL expression in hilar cholangiocarcinoma tissues and cultured cholangiocarcinoma cells, and to assess its ability to induce apoptosis.METHODS: We studied the expression of FasL by human hilar cholangiocaroinomas tissues by immunohistochemistry, and the QBC939 cholangiocarcinomacell line by RT- PCR, immunohiatochemistry, and Western Blot. TUNEL and flow cytometry were used to detect apoptotic cells.RESJLTS: Prevalent expression of FasL was detected in 39resected hilar cholangiocarcinoma tissues. TUNEL staining disclosed a high level of cell death among lymphocytes infiltrating FasL positive areas of tumor. FasL mRNA and protein expressions in cholangiocarcinoma cells could induce Jurkat cells.CONCLUSION: Hilar cholangiocarcinomas may elude immunological surveillance by inducing, via Fas/FasL system, the apoptosis of activated lymphocytes.
出处 《World Journal of Gastroenterology》 SCIE CAS CSCD 2001年第6期860-863,共4页 世界胃肠病学杂志(英文版)
关键词 Immunologic Surveillance Apoptosis CHOLANGIOCARCINOMA Culture Techniques Humans Membrane Glycoproteins RNA Messenger Tumor Cells Cultured cholangiocarcinoma/immunology tumor cells, cultured/ immunology membraneglycoproteins/biosynthesis lymphocytes/immunolo gy apoptosis
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