摘要
用玉米作为受体 ,以水稻、甘蔗、象草、竹子等亲缘较远的植物DNA作为供体 ,用花粉管通道法、种子萌动浸渍法、受体花粉携带法、未成熟籽粒微注射法将供体DNA直接导入受体。发现玉米D1 代在质量性状和数量性状都产生变异 ,而其中以花粉管通道法效果最好 ,种子萌动浸渍法次之 ,注射法最差。
Using corn as the receptor,the DNA of the comparatively far relationstip plants, i e rice, elephant grass, sugarcane and bamboo etc. as the donators, the DNA of the donators were directly derived into the receptor through the methods of pollen tube passage, seed budding immersion, receptor pollen carrying and micro-injection of immature seeds.The results showed that the variations in the D 1 generation of corn took place in the characteristics of quality and quantity. Of all the above mentioned methods the effect of the pollen tube passage method was the best one, the seed budding immersion method was in the second position while the injection method was the worst.
出处
《广西农学报》
2001年第4期16-18,共3页
Journal of Guangxi Agriculture
关键词
外源DNA
直接导入
分子育种
DNA of the Out-Resource
Direct Derivation
Molecular Seeds Breeding
