摘要
[目的]探讨补肾活血法对子宫内膜异位症模型大鼠细胞凋亡基因bcl-2、baxmRNA表达的影响。[方法]将雌性8～12周龄Wister大鼠分为模型组、中药低剂量组、中药高剂量组及西药组。造模成功并分别给药后,采用电镜及RT-PCR法检测药物干预前后模型大鼠异位子宫内膜的病理形态学及凋亡调控基因bcl-2、bax mRNA的阳性表达情况。[结果]补肾活血高剂量组及西药组的异位内膜组织发生凋亡的改变最为明显,并可明显下调bcl-2 mRNA阳性的表达和提升baxmRNA阳性的表达,而中药低剂量组及模型组的改变不明显。[结论]子宫内膜异位症的发生与细胞凋亡能力的下降有关。补肾活血中药能促进内膜细胞发生凋亡,使异位病灶萎缩、消退。
[Objective]To investigate the effect of Reinforcing Kidney and Activating Blood therapy on apoptosis relevant gene bcl-2/bax mRNA expression of rat models with endometriosis.[Methods]Female Wister rats aged 8～12weeks were grouped as model group,Chinese medicine group of low dose,Chinese medicine group of high dose,western medicine group.The electron microscope and RT-PCR staining with specific monoclonal method were adopted to observe the pathomorphology and the positive expression of apoptosis relevant gene bcl-2/bax mRNA.[Results]The apoptosis of endometriosis tissues in the Chinese medicine group of high dose and the western medicine group was the most obvious,and the positive expression of apoptosis regulating gene bcl-2 was downward regulated and that of apoptosis regulating gene Bax was upward regulated.Moreover the model group and the Chinese medicine group of low dose were not obvious.[Conclusion]The occurrence of endometriosis is related with the lowering of ability of apoptotic cells.Chinese medicines with the functions to Reinforcing Kidney and Activating Blood can promote the ability of apoptotic cells in endometriosis to quicken the atrophy and regression of endometriosis tissues.
出处
《浙江中医药大学学报》
CAS
2010年第6期830-832,835,共4页
Journal of Zhejiang Chinese Medical University
基金
2008年上海高校选拔培养优秀青年教师科研专项基金(No:szy 08081)~~
