摘要
In soybean cotyledonary transformation,Agrobacterium infection is influenced by many factors.The cultivation stage of Agrobacterium strain and infectious concentration were studied in this research.Results showed that the highest rate of resistant shoots was obtained when the strain cultivation concentration was OD 600 =0.6,and the infectious concentration was OD 600 =0.5.Seven soybean cultivars in North China were also screened for high susceptibility to Agrobacterium and three genotypes were found to be s...
In soybean cotyledonary transformation,Agrobacterium infection is influenced by many factors.The cultivation stage of Agrobacterium strain and infectious concentration were studied in this research.Results showed that the highest rate of resistant shoots was obtained when the strain cultivation concentration was OD 600 =0.6,and the infectious concentration was OD 600 =0.5.Seven soybean cultivars in North China were also screened for high susceptibility to Agrobacterium and three genotypes were found to be s...
作者
WU Xiaoxia 1,LIU Weiting 2,LI Jing 1,LIU Shanshan 1,WANG Zhikun 1,and LI Wenbin 1 1 College of Agriculture,Northeast Agricultural University,Chinese Education Ministry and Provincial Key Laboratory of Soybean Biology,Harbin 150030,China 2 Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China
基金
Supported by New Variety Breeding Key Project of Genetic Modified Organisms (2008ZX08004-002)
Special Research Fund from Harbin Technology and Science Innovation (2008RFQXN013)
Postdoctoral Fund and Doctoral Fund of Northeast Agricultural University