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Strain Cultivation Stage and Infectious Concentration on Soybean Cotyledonary Node via Agrobacterium–mediated Transformation System

Strain Cultivation Stage and Infectious Concentration on Soybean Cotyledonary Node via Agrobacterium–mediated Transformation System
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摘要 In soybean cotyledonary transformation,Agrobacterium infection is influenced by many factors.The cultivation stage of Agrobacterium strain and infectious concentration were studied in this research.Results showed that the highest rate of resistant shoots was obtained when the strain cultivation concentration was OD 600 =0.6,and the infectious concentration was OD 600 =0.5.Seven soybean cultivars in North China were also screened for high susceptibility to Agrobacterium and three genotypes were found to be s... In soybean cotyledonary transformation,Agrobacterium infection is influenced by many factors.The cultivation stage of Agrobacterium strain and infectious concentration were studied in this research.Results showed that the highest rate of resistant shoots was obtained when the strain cultivation concentration was OD 600 =0.6,and the infectious concentration was OD 600 =0.5.Seven soybean cultivars in North China were also screened for high susceptibility to Agrobacterium and three genotypes were found to be s...
出处 《Journal of Northeast Agricultural University(English Edition)》 CAS 2010年第2期1-6,共6页 东北农业大学学报(英文版)
基金 Supported by New Variety Breeding Key Project of Genetic Modified Organisms (2008ZX08004-002) Special Research Fund from Harbin Technology and Science Innovation (2008RFQXN013) Postdoctoral Fund and Doctoral Fund of Northeast Agricultural University
关键词 soybean cotyledonary node Agrobacterium tumefaciens strain cultivation stage infectious concentration soybean cotyledonary node Agrobacterium tumefaciens strain cultivation stage infectious concentration
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