摘要
目的探讨晚期非小细胞肺癌(NSCLC)组织中DNA修复基因家族成员ERCC1、RRM1和BRCA1的表达及其临床意义。方法应用免疫组织化学方法对122例晚期NSCLC组织进行ERCC1、RRM1及BRCA1蛋白表达检测。回顾性分析NSCLC患者的临床特征、化疗反应及预后与肿瘤组织中ERCC1、RRM1和BRCA1蛋白表达的关系。结果 122例晚期NSCLC组织中ERCC1、RRM1和BRCA1蛋白表达阳性率分别为47.5%、35.2%和46.7%。ERCC1和RRM1蛋白表达阳性率与患者的性别、年龄、吸烟状况、体力状况ECOG评分及肿瘤临床分期和组织学类型均无明显相关性(P>0.05);女性患者肿瘤组织BRCA1蛋白表达阳性率(63.6%)显著高于男性患者(40.4%)。ERCC1和BRCA1蛋白表达阳性与阴性患者的化疗有效率、中位生存期和1年生存率比较差异均无统计学意义(P>0.05);RRM1蛋白表达阳性患者的化疗有效率和1年生存率均显著低于RRM1蛋白表达阴性患者(P<0.05)。结论肿瘤组织中RRM1蛋白表达与晚期NSCLC患者的化疗疗效及预后密切相关。
Objective To investigate the expression of DNA repair gene family members of ERCC1,RRM1 and BRCA1 in tissues of advanced non-small cell lung cancer(NSCLC),and explore its clinical significance. Methods The expression of ERCC1,RRM1 and BRCA1 protein in 122 cases of advanced NSCLC was detected by immunohistochemical methods.The relationship between the expression of ERCC1,RRM1 and BRCA1 protein and the clinical characteristics,response to chemotherapy and prognosis were explored. Results The positive expression of ERCC1,RRM1 and BRCA1 protein in 122 cases was 47.5%,35.2% and 46.7%,respectively.There was no significant correlation between the positive expression of ERCC1 and RRM1 protein and gender,age,smoking status,ECOG physical status scale score,clinical stages and histological types(P>0.05),and the expression of BRCA1 protein in females(63.6%) was significantly higher than that in males(40.4%).There was no significant difference in response rates to chemotherapy,median survival time and 1-year survival rate between patients with positive expression of ERCC1 and BRCA1 protein and those with negative expression of ERCC1 and BRCA1 protein(P>0.05).Patients with positive expression of RRM1 protein had significantly lower response rates to chemotherapy and 1-year survival rate than those with negative expression of RRM1 protein(P<0.05). Conclusion The expression of RRM1 protein in cancer tissues is closely related to the response to chemotherapy and prognosis in patients with advanced NSCLC.
出处
《上海交通大学学报（医学版）》
CAS
CSCD
北大核心
2011年第3期290-294,共5页
Journal of Shanghai Jiao tong University:Medical Science
基金
上海市科委科技发展基金(06DZ19501)
上海市科委学科带头人计划项目(09XD1403500)~~
