Survivin、MMP-2、nm23-H1、VEGF及其受体Flt-1在结直肠癌中的表达及临床意义被引量：12

Expression and clinic value of Survivin,MMP-2,nm23-H1,VEGF and Flt-1 in colorectal carcinomas

下载PDF

导出

摘要背景与目的:近年来的研究表明,多种生物学指标与结直肠癌发生、发展以及手术后的复发转移、预后有关。本研究旨在探讨人结直肠癌组织中Survivin、MMP-2、nm23-H1、VEGF及其受体Flt-1的表达与各临床病理参数及预后的关系及临床意义。方法:采用免疫组织化学SP法,检测72例结直肠癌组织及其对照癌旁正常黏膜组织中Survivin、MMP-2、nm23-H1、VEGF及其受体Flt-1表达的状况,分析其与临床病理参数及复发转移的关系。结果:在结直肠癌中Survivin、MMP-2、nm23-H1、VEGF及其受体Flt-1的表达率分别为62.5%(45/72)、66.7%(48/72)、55.5%(40/72)、61.1%(44/72)、79.2%(57/72),均显著高于对照癌旁正常黏膜组织(P<0.01)。Survivin表达与浸润深度、淋巴结转移、TNM分期及术后复发转移有关;MMP-2表达与淋巴结转移、TNM分期、术后复发转移有关;nm23-H1表达与淋巴结转移、TNM分期相关;VEGF表达与浸润深度、TNM分期及术后复发转移有关;而受体Flt-1表达与临床病理及预后因素均无关。结论:Survivin、MMP-2、nm23-H1、VEGF与结直肠癌的发生、发展密切相关,联合检测多个相关基因的表达能更准确地判断结直肠癌的生物学特征及预后判断。 Background and purpose:The aim of this study was to investigate the relationship between the expression of Survivin,MMP-2,nm23-H1,VEGF,Flt-1 and clinicopathologic parameters in colorectal carcinoma(CRC).Methods:The expression of Survivin,MMP-2,nm23-H1,VEGF and Flt-1 were detected using the immunohistochemical method in 72 CRCs and their match normal tissue around tumor.The correlations between the expression of Survivin,MMP-2,nm23-H1,VEGF,Flt-1 and clinicopathologic parameters were analyzed.Results:The positive rates for Survivin,MMP-2,nm23-H1,VEGF and Flt-1 were 62.5%,66.7%,55.6%,61.1% and 79.1%,respectively,and they were all significantly higher than those in matching non-carcinomatous ends of cut specimen.The expression of Survivin in CRCs was correlated with the invasive depth of the tumor,lymphatic metastasis,TNM staging,postoperative recurrence and metastasis.The expression of MMP-2 in CRCs was correlated with lymphatic metastasis,TNM staging,postoperative recurrence and metastasis.The expression of nm23-H1 in CRCs was correlated with lymphatic metastasis and TNM staging.The expression of VEGF in CRCs was correlated with invasive depth of tumor,TNM staging,postoperative recurrence and metastasis.Conclusion:Survivin,MMP-2,nm23-H1 and VEGF are correlated closely with the development and progression of CRCs.It might be helpful to evaluate the biological properties and prognosis of CRCs more accurately through using a combined analysis of the expressions in multiple genes.

作者陈中皓王学志徐铭杨华黄忠华

机构地区上海市长宁区中心医院胃肠外科上海市长宁区中心医院病理科上海市长宁区中心医院化验科

出处《中国癌症杂志》 CAS CSCD 北大核心 2011年第4期290-293,共4页 China Oncology

关键词结直肠癌 SURVIVIN MMP-2 NM23-H1 VEGF FLT-1 Colorectal cancer Survivin MMP-2 nm23-H1 VEGF Flt-1

分类号 R73 [医药卫生—肿瘤]

引文网络
相关文献

参考文献16

1Masaubmi Shibuya.Vascular endothelial growth factor receptor-1 (VEGFR-1/Flt-1): a dual regulator for angiogenesis[J]. Angiogenesis . 2006 (4)
2Ambrosini G,Adida C,Sirugo G,et al.Induction of apoptosis and inhibition of cell proliferation by survivin gene targeting. Journal of Biological Chemistry . 1998
3Vihinen P,Kahari V M.Matrix metalloproteinases in cancer: prognostic markers and therapeutic targets. International Journal of Cancer . 2002
4Kraiem Z,Korem S.Matrix metalloproteinases and the thyroid. Thyroid . 2000
5Sun J,Hemler ME.Regulation of MMP-1 and MMP-2 production through CD147 extracellular matrix metalloproteinase inducer interactions. Cancer Research . 2001
6Steeg P S,Bevilacqua G,Kopper L,et al.Evidence for a novel gene asociated with low tumor metastatic potential. Journal of the National Cancer Institute . 1988
7Steeg P S,Cohn K H,Leone A,et al.Tumor metastasis and nm23: current concepts. Cancer Cell . 1991
8MacDonald N J,De la Rosa A,Benedict M A,et al.A serine phosphorylation of nm23, and not its nucleoside diphosphate kinase activity, correlates with suppression of tumor metastatic potential. Journal of Biological Chemistry . 1993
9Leone A,McBride OW,Weston A,et al.Somatic allelic deletion of nm23 in human cancer. Cancer Research . 1991
10Berney C R,Fisher R J,Yang J,et al.Protein markers in colorectal cancer: predictors of liver metastasis. Annals of Surgery . 1999