摘要
用正交试验法研究了嗜线虫致病杆菌北京变种菌株CB6的营养利用以及培养基成分的改变对产生杀虫活性物质的影响。通过筛选该菌对碳源、氮源以及无机盐的需求,确定了该菌的最佳发酵培养基为(g/L):豆粉40g,蔗糖15g,蛋白胨15g,酵母膏10g,玉米浆5g,NaH2PO41g,NaCl5g,谷氨酸5g。培养液上清液对玉米螟初孵幼虫的体重抑制率为90 81%,比基础培养基的杀虫活性提高了46.5%。
The culture medium of Xenorhabdus nematophila var. pekingensis was improved based on tests of nutrition requirements. The result showed that fructose, glutamic acid and phosphate increased insecticidal activity. The optimum culture media was (g/L): soybean flour 40, sucrose 15, peptone 15, yeast extract 10, maize slurry 5.0, sodium chloride 5.0, phosphate 1.0, glutamic acid 5.0. The fermentation filtrate of X. nematophila var. pekingensis cultured in optimum medium displayed insecticidal activity. Rate of growth inhibition of neonate larvae of Ostrinia furnacalis was 90.81%. It was increased by 46.5% in comparison with basic culture medium (TSB).
出处
《中国生物防治》
CSCD
北大核心
2004年第2期118-121,共4页
Chinese Journal of Biological Control
基金
国家自然科学基金(30070519)
农业部农作物病虫草害生防资源与利用重点实验室基金(LOBCR 01 03)
关键词
嗜线虫致病杆菌北京变种
培养基
杀虫活性
Xenorhabdus nematophila var. pekingensis
insecticidal activity
culture medium
