摘要
Objective;In vitro studies have shown that KGF-2 has a proliferative effect on neonatal foreskin kerati-nocytes.Cells from adult donors have been shown to respond to KGF-1 to a lesser degree than neonatal keratino-cytes.The purpose of the study was to investigate the proliferative effect of KGF-2 on keratinocytes from an adultsubject.Methods;Standard medium was Keratinocyte Growth Medium without BPE,hydrocortisone and EGF.Ke-ratinocytes cultured from a 48-year-old subject were seeded at 2 10~4 in 32 mm ...
Objective;In vitro studies have shown that KGF-2 has a proliferative effect on neonatal foreskin kerati- nocytes.Cells from adult donors have been shown to respond to KGF-1 to a lesser degree than neonatal keratino- cytes.The purpose of the study was to investigate the proliferative effect of KGF-2 on keratinocytes from an adult subject.Methods;Standard medium was Keratinocyte Growth Medium without BPE,hydrocortisone and EGF.Ke- ratinocytes cultured from a 48-year-old subject were seeded at 2 10~4 in 32 mm dishes in standard medium with EGF. After 24 hours,medium was changed to standard medium with KGF-2 at 4,16,125 and 500 ng/ml or without KGF-2.Standard medium with EGF was used as a positive control.The growth of keratinocytes was monitored by the MTT assay and by photographs at day 3,5 and 7.Each treatment was performed in triplicate.Results;KGF-2 concentrations from 4 to 500 ng/ml all showed a significant proliferative effect on days 5 to 7 compared to the nega- tive control (P<0.01).By day 3 the response of keratinocytes in KGF-2 was 1.5 to 2.5-fold,by day 5 was 3 to 5- fold and by day 7 was 3 to 12-fold relative to the negative control.The optimal response was in 125 ng/ml KGF-2 at day 7.Cell proliferation was also consistently higher in all KGF-2 concentrations compared to the positive control. Conclusion;KGF-2 exerted a significant effect on the proliferation of adult keratinocytes,which was higher than that of the EGF concentration in the growth medium.This supports the clinical work where KGF-2 has been shown to improve the healing in chronic wounds in adults.
出处
《感染．炎症．修复》
2002年第2期74-77,共4页
Infection Inflammation Repair
