摘要
目的 分析慢性乙型肝炎(chb)患者外周血单个核细胞(pbmc)中cd8+t细胞免疫球蛋白和黏蛋白结构域分子-3(tim-3)的表达及其意义.方法 用流式细胞术检测58例chb患者和16名健康体检者pbmc中cd8+t细胞tim-3的表达水平.采用酶联免疫斑点试验(elispot)检测tim-3单克隆抗体阻断tim-3/tim-3l通路前后hla-a2阳性患者pbmc中产生ifnγ的hbv特异性细胞毒性t淋巴细胞(ctl)数量的改变.阻断前后斑点数量的比较采用配对t检验,定量资料用spearman非参数检验进行相关性分析.结果 chb患者pbmc中cd8+t淋巴细胞tim-3分子表达水平为(14.2±8.98)％,明显高于健康体检者的(4.80±2.92)％,差异有统计学意义(x2=92.48,p＜0.05).16例重度chb患者和42例轻度chb患者cd8+t细胞tim-3表达分别为( 19.54±10.95)％和(9.58±7.30)％,差异有统计学意义(x2=77.24,p＜0.05).tim-3单克隆抗体阻断tim-3通路前,chb患者pbmc中产生ifnγ的hbv特异性ctl数量为7.27 ±3.14,阻断后为19.62 ±4.97,差异有统计学意义(t=2.95,p ＜0.05).结论 chb患者pbmc中cd8+t细胞高表达tim-3分子抑制了hbv特异性ctl功能,阻断tim-3通路可以促进表达ifnγ的hbv特异性ctl增殖,增强抗病毒效应.
objective to investigate the expression of t cell immunoglobulin-and mucin-domaincontaining molecule-3 (tim-3) in peripheral cd8 +t cells and its significance in patients with chronic hepatitis b (chb).methods fifty-eight chb patients and 16 healthy controls were enrolled.tim-3 expression in cds + t cells was detected by flow cytometry,and quantities of ifnγ-producing hbv-specific cytotoxic t lymphocytes (ctls) in hla-a2 positive subjects were detected by enzyme-linked immunosorbent spot (elispot) test before and after the blockade of tim-3/tim-3l pathway.paired t test was performed to compare the quantities of ctls before and after the blockade,and nonparametric spearman correlation analysis was performed to explore the correlation in quantitive data.results tim-3 expression in chb patients was (14.2 ± 8.98 )％,which was higher than that of healthy controls (4.80 ± 2.92)％,and the difference was of statistical significance (x2 =92.48,p ＜ 0.05 ) tim-3 expressions in 16 severe chb patients and 42 mild chb patients were ( 19.54 ± 10.95) ％ and (9.58 ± 7.30) ％,respectively,and the difference was statistically significant (x2 =77.24,p ＜ 0.05 ). before the blockade of tim-3/tim-3l pathway,ifnγ-producing hbv-specific ctls were 7.27 ± 3.14,and it increased to 19.62 ± 4.97 after the blockade ( t =2.95,p ＜ 0.05 ).conclusion the upregulation of tim-3 on peripheral cd8 + t cells may inhibit hbv-specific ctls,and the blockade of tim-3 pathway can enhance the proliferation of ifnγ-producing hbv-specific ctls,thus can enhance antiviral effect.
出处
《中华临床感染病杂志》
CAS
2012年第1期33-36,23,后插1－后插2,共4页
Chinese Journal of Clinical Infectious Diseases
