摘要
本试验比较了几种提取微生物固体发酵代谢物的方法,旨在筛选出一种尽可能多保留了代谢物的方法。试验采用热带假丝酵母固体发酵棉粕源底物,以液相色谱串联质谱(LC-MS)为分析平台,分别采用50%冷甲醇溶液(甲醇法)、酸性甲醇-乙腈溶液(酸性甲醇-乙腈法)和50%乙腈溶液(乙腈法)提取发酵代谢物。结果表明:采用酸性甲醇-乙腈法、乙腈法和甲醇法分别提取出1 831、1 522和1 262种代谢物,提取效率表现为酸性甲醇-乙腈法>甲醇法>乙腈法。本试验中3种方法相比,酸性甲醇-乙腈法提取的代谢物种类最多,提取效率最佳,是微生物固体发酵代谢物提取的有效方法。
In order to find a good extraction method that can keep metabolites as many as possible,several extraction methods for solid fermentation metabolites were compared in this study.Substrates with cottonseed meal were fermented by Candida tropicalis,and the analysis of metabolites was carried out using the liquid chromatographic-mass spectrometry(LC-MS) platform.Three different extraction solutions,including 50% cold methanol solution(MeOH method),acidic methanol-acetonitrile solution(AMF method) and 50% acetonitrile solution(MeCN method),were used.The results showed that the number kinds of metabolites extracted by methods of AMF,MeCN and MeOH were 1 831,1 522 and 1 262,respectively;extraction efficiency was shown as AMF method>MeOH method>MeCN method.Among the three methods in this experiment,AMF method is an effective method for metabolites extraction in solid fermentation,and acts better on both kinds of metabolites and extraction efficiency.
出处
《动物营养学报》
CAS
CSCD
北大核心
2012年第8期1610-1614,共5页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
国家自然科学基金项目(30960258)
石河子大学科技成果转化引导资金专项(kjkfyzh2009-cgzh01)
关键词
热带假丝酵母
固体发酵
发酵饲料
代谢物
提取方法
Candida tropicalis
solid fermentation
fermented feed
metabolites
extraction methods
