摘要
以玻璃纤维织物为载体,通过浸渍法对其表面进行偶联剂改性制备生物固定化载体,并对脂肪酶进行了固定化。考察了偶联剂种类、溶液浓度及浸渍时间对其表面改性的影响。利用接触角测量、红外光谱分析及脂肪酶固载率的测定,对玻璃纤维表面改性效果及表面官能团变化情况进行了研究。结果表明,玻璃纤维偶联剂改性制备的固定化载体表面润湿性能明显改善,对脂肪酶的固载率明显提高,硅烷偶联剂种类、浓度及浸渍时间对玻璃纤维表面性能都有不同程度影响,当偶联剂体积分数为3%、浸渍时间为10min时,改性效果在本实验条件下最明显。
Immobilized carrier was prepared by the dipping method using silane coupling agent on the surface of glass fiber cloth.Effects of reaction time,kind and concentration of silane coupling agents on the properties of carrier were systematically studied.FT-IR spectrums were employed to analyze the surface component.Determination of contact angle and lipase immobilization efficiency before and after grafting modification show that properties of modified glass fiber are better than that of unmodified.The optimal modifying condition is as follows: the content of silane coupling agent is 3%,the dipping time is 10 min.
出处
《大连工业大学学报》
CAS
北大核心
2012年第3期222-225,共4页
Journal of Dalian Polytechnic University
基金
辽宁省教育厅创新团队项目(2006T030)
辽宁省教育厅科学研究计划资助项目(2004D006)
关键词
固定化载体
玻璃纤维
硅烷偶联剂
表面改性
immobilized carrier
glass fiber
silane coupling agent
surface modification
