摘要
目的:观察大鼠肢体缺血再灌注后关节软骨中CXC趋化因子受体4和基质金属蛋白酶-9(matrix metal-loproteinase,MMP-9)的表达,探讨其在关节软骨损伤中的意义。方法:采用Wistar大鼠股动脉夹闭法建立肢体缺血再灌注损伤模型,将40只大鼠随机分成正常对照组(NG)、单纯缺血组(IG)、缺血再灌注1周组(IR1G)、缺血再灌注3周组(IR3G)和缺血再灌注8周组(IR8G),分别在术后1,3,8周处死实验动物,进行大体标本观察,用蕃红O染色观察膝关节软骨蛋白多糖(proteoglycan,PG)的变化,用免疫组织化学S-P法分别测定CXCR4和MMP-9在关节软骨中不同时相的表达。结果:缺血再灌注后,随时间延长,关节软骨中的蛋白多糖含量逐渐降低,CXCR4、MMP-9的表达均明显增强,但在8周时降低(P<0.01)。结论:CXCR4联合MMP-9的表达升高可能是导致缺血再灌注后关节软骨损伤的重要因素之一。
Objective To investigate the expression and significance of CXCR4 and matrix metalloproteinase 9(MMP-9) in rats articular cartilage following ischemia/reperfusion injury.Methods Wistar rats were used to establish ischemia reperfusion model.Forty rats were randomly divided into three groups: normal control group(NG),ischemia group(IG) and ischemia-reperfusion group(IRG).The expression of CXCR4 and MMP-9 was detected by immunohistochemical method and analyzed semi-quantively at different time points following ischemia reperfusion.The pathological changes of articular cartilages were also observed and the proteoglycans(PG) were detected.Results The expression of PG decreased as time went by.CXCR4,MMP-9 expressed higher in ischemia/reperfusion rats compared to control group,and increased more with time extension(P<0.01).However,both CXCR4 and MMP-9 decreased in 8 weeks.Conclusion CXCR4 and MMP-9 are probably important factors resulting in articular cartilage injury following ischemia-reperfusion.
出处
《湖北医药学院学报》
CAS
2012年第2期119-122,181,共5页
Journal of Hubei University of Medicine
