摘要
目的:观察二黄解郁汤对肝癌细胞生长抑制作用及对MMP-2表达的影响。方法:分别用不同浓度二黄解郁汤作用不同时间于人肝癌HepG2细胞,以MTT法观察并计算IC50值,荧光显微镜检测HepG2细胞生长情况,Western blot检测HepG2细胞中MMP-2蛋白的表达。结果:二黄解郁汤对HepG2细胞作用24,48和72 h的IC50分别为600,550,150μg/mL;荧光显微镜下可见细胞内药物绿色荧光,且细胞数量随药物浓度增加而减少;Westernblot结果表明,经100,200,400μg/mL的二黄解郁汤干预的细胞中MMP-2蛋白的表达分别占空白组的(77.3±4.84)%、(56.25±3.31)%、(33.21±7.26)%,呈剂量依赖性下降(P<0.01)。结论:二黄解郁汤对肝癌细胞具有明显的抑制作用,其作用可能与抑制MMP-2蛋白的表达有关。
Objective To investigate the inhibitory effect of cell proliferation and expression of MMP-2 by Erhuang Jieyu Tang in HepG2 cells.Methods A serious concentration of 0,40,80,100,200,400,800 μg/mL of Erhuang Jieyu Tang were administered on human hepatocellular carcinoma HepG2 cells for 24,48,72 h,IC50 value was detected by MTT.The viability of HepG2 cells with different concentration of Erhuang Jieyu Tang(0,100,200,400 μg/mL) for 48 h was detected by fluorescence microscopy directly;the expression of MMP-2 in HepG2 with Erhuang Jieyu Tang was determined by Western blot.Results The IC50 value of HepG2 cells treated with Erhuang Jieyu Tang for 24 h,48 h and 72 h were 600,550,150 μg/mL,respectively.The viable cells were dramatically decreased;and data also showed that compared with cells without treated,the rate of level of MMP-2 in HepG2 with Erhuang Jieyu Tang were(77.3±4.84)%,(56.25±3.31)%,(33.21±7.26)%,in a dose-dependent manner(P<0.01).Conclusion Erhuang Jieyu Tang could inhibit the proliferation of HepG2 cells,which may correlate with the inhibitory effect on of MMP-2 protein expression.
出处
《湖北医药学院学报》
CAS
2012年第1期46-48,封3,共4页
Journal of Hubei University of Medicine
关键词
二黄解郁汤
肝癌
迁移
MMP-2
Erhuang jieyu tang
Hepatocellular carcinoma
Metastasis
MMP-2
