摘要
[目的]观察附子提取物诱导胃癌SGC-7901细胞凋亡的作用及机制。[方法]分别采用不同浓度(20、40、80 mg·mL^(-1))的附子溶液干预SGC-7901胃癌细胞24h、48h、72h,用四唑盐(MTT)比色法检测附子提取物对肿瘤细胞的抑制作用:采用以上浓度梯度的附子干预SGC-7901细胞24h(或采用80 mg·mL^(-1)的附子干预0h、24h、48h),应用Annexin V-FITC/PI双重染色,通过流式细胞仪观察SGC-7901的凋亡情况。[结果]附子提取物能显著地抑制SGC-7901细胞的生长,并具有浓度及时间依赖性,细胞有明显的凋亡改变。[结论]附子提取物具有抗胃癌SGC-7901细胞增殖和诱导癌细胞凋亡的作用。
[Purpose]The apoptosis of SGC-7901 gastric cancer cells induced by Aconite root were studied in order to assess its antitumor effect. [Methods]The depressant effect of SGC-7901 gastric cancer cell as studied based on MTT assay in vitro and treated by Aconite root of 20,40,and 80mg/mL concentrations for 3 days.Quantity of apoptosis was tested by the same concentrations for 1 day,or was studied by 80mg/mL Aconite root for 0,24,and 48 hours.That were observed and analyzed by flow cytometry with Annexin V and propidium iodide double staining.[Results]The proliferation of SGC-7901 cells was obviously inhibited by Aconite root on dose dependent and time dependent manne and apoptosis of SGC-790 1 was induced by Aconite root.[Conclusions]Based on the above resuXts suggest that Aconite root was able to inhibit the growth and induce the apoptosis of SGC-7901 gastric cancer cells.
出处
《浙江中医药大学学报》
CAS
2011年第5期665-668,共4页
Journal of Zhejiang Chinese Medical University
基金
苏州市社会发展科技计划项目(NO:SZ0854)~~
