摘要
以黑腹果蝇(Drosophila melanogaster)为试验动物,研究了亚硝酸钠对果蝇寿命和生育力的影响及维生素C(Vc)对其拮抗作用。结果表明:培养基中添加亚硝酸钠可以显著缩短果蝇的平均寿命、最短和最长平均寿命。与对照组相比,除30mg/mL亚硝酸钠处理组的雄果蝇差异不显著外,其他实验组果蝇平均寿命均显著或极显著缩短(P<0.05或P<0.01)。果蝇的生育力也随着亚硝酸钠浓度的升高而降低,各实验组与对照相比差异均达到极显著水平(P<0.01)。随后在含480mg/mL亚硝酸钠的培养基中加入不同浓度Vc,结果发现,除雄果蝇的0.13%实验组与对照差异不显著外,其他各组雌、雄果蝇的平均寿命随Vc浓度的提高而显著或极显著延长(P<0.05或P<0.01)。同样,Vc浓度为0.40%和1.20%实验组果蝇生育力亦极显著高于对照组。
In this study,Drosophila melanogaster was used as the experimental animal to study the effects of sodium nitrite on life-span,reproductive capacity and antagonism of Vc on sodium nitrite.The results showed that the life-span,average longest and average shortest life-span was shortened in sodium nitrite.Compared with the control group,the average life-span of D.melanogaster was shortened in sodium nitrite significantly (P < 0.05 or P < 0.01) except for 30 mg/mL sodium nitrite experimental group.The reproductive capacity of D.melanogaster reduced in sodium nitrite significantly (P < 0.01).When the concentration of Vc were 0.13%,0.40% and 1.20% in the 480 mg/mL sodium nitrite Medium,with the concentration of Vc increased,the average life span of D.melanogaster was prolonged significantly(P < 0.05 or P < 0.01) except for 0.13% Vc experimental group of male D.melanogaster.The reproductive capacity of D.melanogaster was increased significantly (P < 0.01) in 0.40% and 1.20% Vc experimental group.
出处
《中国农学通报》
CSCD
北大核心
2011年第2期400-404,共5页
Chinese Agricultural Science Bulletin
基金
河北大学青年基金
保定市科学技术研究与发展指导计划项目(10ZF002)
关键词
亚硝酸钠
果蝇
寿命
生育力
VC
sodium nitrite
Drosophila melanogaster
life span
reproductive capacity
Vc