摘要
为探讨游泳应激对小鼠心肌和腿肌抗氧化能力的影响,试验选取50只小鼠进行游泳试验,分别于游泳前、游泳10、20、30、50 min后随机选取10只小鼠眼眶采血致死,采集心肌和腿肌,测定样品中MDA含量、SOD和GSH-Px活性。结果表明,10 min游泳应激后,小鼠心肌MDA含量显著增加(P<0.05),20min和30 min后轻微下降,50 min后又显著上升(P<0.05),游泳过程中小鼠心肌SOD和GSH-Px活性均无显著变化。游泳过程中小鼠腿肌MDA含量均无显著变化,10 min和20 min游泳应激后,腿肌SOD活性显著降低(P<0.05),之后有所回升,50 min后SOD活性仍显著低于对照组(P<0.05)。游泳过程中腿肌GSH-Px活性持续升高,且40 min组显著升高(P<0.05)。
In order to research the effects of swimming stress on lipid peroxidation of hearts and leg muscles in mice,50 Kunming mice were used in the swimming experiment.10 mice selected randomly were sacrificed before swimming and afters swimming for 10,20,30,50 min respectively.Hearts and leg muscles of mice were collected,of which maleic dialdehyde(MDA) content,superoxide dismutase(SOD) activity and glutathione peroxidase(GSH-Px) activity were determined by spectrophotometry.The results showed that MDA content in heart tissue of mice after swimming for 10 min was increased significantly(P<0.05),decreased slightly after swiming for 20 and 30 min and increased markedly after swimming for 50 min(P<0.05).SOD and GSH-Px activity of heart tissue had no significant change during the whole swimming progress;MDA content of leg muscles had no significant change during the whole swimming progress.SOD activities of leg muscles after swiming for 10 and 20 min significantly decreased(P<0.05) and then rised slightly,SOD activity of the 50 min group was still lower than that of the control group(P<0.05).GSH-Px activity of leg muscle during the swimming process continued to rise and increased markedly in the 40 min group(P<0.05).
出处
《湖北农业科学》
北大核心
2012年第22期5131-5133,共3页
Hubei Agricultural Sciences
基金
中国博士后科学基金项目(2011M500937)
江苏省博士后科研资助计划项目(1102078C)
关键词
游泳应激
小鼠
心肌
腿肌
脂质过氧化
swimming stress
mice
heart muscles
leg muscles
lipid peroxidation
