摘要
从湖北省武汉郊区和监利市两地的发病雏鸭分离纯化到4株鸭疫里默氏杆菌(Riemerella a-natipestifer,RA),通过对该菌形态学鉴定、生化试验、细菌16S rRNA的快速鉴别诊断证明了4株细菌均为鸭疫里默氏杆菌。将不同来源的菌株各选一株HBSPX和HBJL,进行了16S rRNA目的片段的扩增并测序,测序结果表明这两株细菌与其他鸭疫里默氏杆菌的16S rRNA同源性高达99.4%和98.9%。药敏试验结果表明,HBSPX株对阿莫西林、先锋VI高度敏感,但HBJL株对氯洁霉素和红霉素高度敏感。
Four strains isolated from diseased duck in Wuhan suburbs and Jianli of Hubei province were identified as Riemerella anatipestifer(RA) by morphological identification,biochemical tests and PCR identification of 16 S rRNA of bacteria.16 S rRNA gene of bacteria was PCR amplified from two isolated strains HBSPX and HBJL.The nucleotide sequence identity of 16 S rRNA between two isolated strains and other RA was 99.4% and 98.9%.In drug sensitive test,HBSPX was highly sensitive to amoxicillin and cephalexin;and HBJL was highly sensitive to clindamycin and erythromycin.
出处
《湖北农业科学》
北大核心
2012年第22期5193-5196,共4页
Hubei Agricultural Sciences
基金
公益性行业(农业)科研专项(201003012)
湖北省农业科技创新中心项目(2011-620-001-003)
动物胚胎工程及分子育种湖北省重点实验室开放课题项目(2010ZD150)
