摘要
目的观察耐力训练及力竭运动后大鼠大脑海马区锥体细胞及其线粒体的超微结构变化。方法实验于2007年6月至2008年11月在郑州大学完成。选取8周龄雄性SD大鼠40只,随机设耐力训练组:安静组;急性力竭运动后24h组;耐力训练+急性力竭运动后即刻组;耐力训练+急性力竭运动后24h组。每组10只。安静组不外加运动,其他组次日进行力竭运动,力竭运动开始的速度为10m/min,逐渐提高速度并在3min内到达预定的速度(中等强度、大强度力竭运动的速度分别为20m/min、36m/min),保持速度直至力竭,并记录力竭运动时间。耐力训练方案:大鼠在动物跑台进行运动训练,1次/d,6d/周。跑台速度由开始的10m/min逐渐增加至第4周30m/min,运动时间由30min/d增加到40min/d。力竭标准为大鼠用毛刷驱赶无效,在跑台尾端停留2s仍不愿跑,且失去快速翻正反射。主要观察指标:断头处死分别取材检测大鼠大脑海马区锥体细胞及其线粒体的超微结构变化。结果 40只SD大鼠均完成实验设计方案,全部进入结果分析。结果发现耐力训练和力竭运动后大鼠大脑细胞凋亡数量显著增加,力竭运动强度增加,凋亡细胞数量增多,且多为神经胶质细胞,安静组大脑细胞凋亡率为(6.56±1.24)%、急性运动后24h组为(16.14±3.26)%、耐力训练+急性运动后即刻组为(29.78±1.96)%、耐力训练+急性运动后24h组为(32.43±2.35)%。通过图像分析系统的分析研究,海马神经元线粒体变性较为显著。结论本实验观察到耐力训练和力竭运动对大脑细胞造成一定的损伤,海马区神经元线粒体变性,可能是由于疲劳训练引起脑组织的酸中毒和缺氧引起大脑细胞的一些变性现象。
Objective The pyramid neurons in the CA1 subfield of the hippocampus are vulnerable to ischemic attack,and transient global ischemia can lead to a specific neuronal death called the delayed neuronal death.To analyze the effect of changes of pyramidal cell and mitochondria by transmission electron microscopy in rats′ hippocampus after endurance training and exhaustion treadmill running.Methods The experiment was done from June 2007 to November 2008 in Zhengzhou University.Forty male SD rats of 8 weeks old were selected and divided into 4 groups randomly with 10 rats in each group:sedentary group(without training),24 hours after acute exercise group(without training,sacrificed 24 hours later after acute exhaustion treadmill running),right after acute exercise with training group(performance endurance training program,rats were sacrificed right after acute exhaustion treadmill running),24 hours after acute exercise with training group(performance endurance training program,rats were sacrificed 24 hours after acute exhaustion treadmill running).Endurance training program:once a day,6 days a week.Speed increased from 10 meters per minute at the first week to 30 meters per minute at the fourth week.Training time was that from 30 minutes per day at the first week to 40 minutes per day at the fourth week.Acute exercise means an exhaustion treadmill running with the speed of 28 meters per minute.The exhaustion standard was the rat could not keep the speed and stagnant stay in the 1/3 back part of the treadmill even with electricity stimulation for 10 s more than 3 times.Rats were sacrificed with decollation.Hippocampus of the brain were separated were used to detect the changes on cell apoptosis of brain and observation of pyramidal cell and mitochondria by transmission electron microscopy in rats′ hippocampus after endurance training and exhaustion treadmill running.Results Totally 40 SD rats were done the experimental program and involved in the result analysis.The results showed that the percentage of apoptotic cells in brain and the degeneration of pyramidal cell and mitochondria by transmission electron microscopy in rats′ hippocampus increased significantly after endurance training and exhaustion treadmill running.And that the apoptotic cells were all neuroglials.Conclusions It was concluded that the endurance training and exhaustion treadmill running could infect the cell function of brain especially the function of study and memory.
出处
《中华临床医师杂志（电子版）》
CAS
2012年第18期46-49,共4页
Chinese Journal of Clinicians(Electronic Edition)
关键词
细胞凋亡
锥体细胞
线粒体
耐力训练
力竭运动
Apoptosis
Pyramidal cells
Mitochondria
Endurance training
Exhaustion treadmill running
