摘要
利用RT-PCR和RACE技术克隆得到鲤鱼(Cyprinus carpio L.)X盒结合蛋白1(Xbp1)基因编码序列全长。生物信息学分析发现,鲤鱼Xbp1 mRNA的26 bp内含子及其两侧序列能形成典型的茎环结构,其蛋白结构具有1个保守的碱性亮氨酸拉链(bZIP)结构域;系统进化分析表明鲤鱼Xbp1与斑马鱼的亲缘关系最近;实时荧光定量PCR检测结果表明,两种亚型Xbp1 mRNA在鲤鱼不同组织中广泛表达,且未剪接型Xbp1 mRNA的表达量明显高于剪接型。
We cloned the full-length cDNA of X-box binding protein 1(Xbp1) in Cyprinus Carpio L.with RT-PCR and RACE.A conserved stem-loop structure formed by 26 bp intron and its flanking sequence in Xbp1 mRNA and a basic region leucine zipper(bZIP)domain in its protein structure are identified by bioinformation analysis.Phylogenetic analyses show that the carp Xbp1 has the closest relationship with zebrafish Xbp1.Real-time PCR analyses reveal ubiquitous expression of two subtypes of Xbp1 in different tissues of carp.Additionally,the main expression is unspliced Xbp1 mRNA rather than the spliced one under normal conditions.
出处
《济南大学学报（自然科学版）》
CAS
北大核心
2013年第2期163-168,共6页
Journal of University of Jinan(Science and Technology)
基金
济南市高校自主创新计划(200906020)
山东省自然科学基金(Y2007D37)
