摘要
目的研究Smac和LIVIN在人体胃癌组织中的表达,初步探讨其与胃癌的发生、癌细胞增殖和侵袭转移的关系及其临床意义。方法采用链霉素抗生物素蛋白-过氧化酶连接(SP)免疫组织化学方法检测75例胃癌标本粘膜组织中Smac和LIVIN蛋白的表达,胃溃疡或十二指肠溃疡患者胃粘膜组织作为正常对照组。结果胃癌组织中LIVIN的表达率显著高于对照胃粘膜组织,Smac的表达率则低于对照胃粘膜组织,差异均有统计学意义(P<0.01);LIVIN与Smac的表达呈负相关(P<0.001,r=-0.720、P<0.001,r=-0.336),二者的阳性表达率与肿瘤淋巴转移、临床分期、肿瘤病理分化程度及浸润深度密切相关,与性别、年龄及肿瘤大小无关(P>0.05)。结论LIVIN与Smac在胃癌的发生发展及浸润转移中存在着相互制约的关系。检测两者在胃癌中的表达,有助于从不同角度判断胃癌的恶性生物学行为;LIVIN和Smac有可能成为胃癌临床诊断、判断疗效及监测预后的可靠指标。
【Objective】To study the expression of Smac and LIVIN in human gastric cancer and evaluate their effects on carcinogenesis,cell proliferation,invasion and metastasis of gastric carcinomas,as well as their clinical significance. 【Methods】The expression of Smac and LIVIN was detected by immunohistochemical method (strepta-vidin-peroxidase) in the tissues of 75 primary gastric carcinomas and 15 gastric ulcers. 【Results】The positive ex-pression rate of LIVIN in gastric carcinoma tissues was significantly higher than that in normal tissues,and the ex-pression of Smac was obviously lower than that in normal tissues (P <0.01). The expression of LIVIN was negatively correlated with the expression of Smac (P <0.001,r =-0.720; P <0.001,r =-0.336). The expression of Smac and LIVIN was closely correlated with lymph node metastasis,clinical stage,histological differentiation grade and tumor depth of gastric cancer,but not correlated with sex,age and tumor size (P >0.05). 【Conclusion】LIVIN and Smac have mutual restrictive relationships in the process of invasion and metastasis of gastric cancer. Detecting the ex-pression of Smac and LIVIN in gastric cancer is helpful to judge the malignant biological behavior of gastric cancer. LIVIN and Smac may be considered as reliable markers for clinical diagnosis,therapy efficacy and the prognosis of gastric carcinoma.
出处
《中国医学工程》
2008年第4期254-259,共6页
China Medical Engineering
