摘要
目的建立鸡肉中二氯二甲吡啶酚残留量的气相色谱-质谱分析方法.方法样品经甲醇提取,中性氧化铝柱净化,在碱性条件下以吡啶催化,丙酸酐衍生后,用气相色谱-质谱仪测定.结果二氯二甲吡啶酚在20~240μg/L浓度范围内与响应值呈良好的线性关系,线性回归方程为Y=1.132X?2.553,相关系数大于0.998.该方法的平均回收率为78.7%~85.1%,相对标准偏差(RSD)为7.8%~12.6%,检出限(LOD)为:鸡肉5.0μg/kg,鸡肝10.0μg/kg.结论该方法选择性好,适合鸡肉中二氯二甲吡啶酚残留量的测定.
Objective To establish a method for the determination of dichloro dimethyl pyridine phenol residues in chicken tissues by gas chromatography-mass spectrometry (GC-MS). Methods Dichloro dimethyl pyridine phenol was extracted with methanol and cleaned-up though Al2O3 column and derived with propionic anhydride under alkaline conditions using pyridine as catalyst. Quantification was achieved by GC-MS. Results The recoveries of dichloro dimethyl pyridine phenol in chicken tissues were in the range of 78.7%~ 85.1% with the relative standard deviation (RSD) of 7.8%~12.6%. The limit of detection (LOD) was 5.0 μg/kg for chicken and 10.0 μg/kg for chicken livers. Conclusion The method has good selectivity and it is suitable for the determination of dichloro dimethyl pyridine phenol residues in chicken tissues.
出处
《食品安全质量检测学报》
CAS
2013年第1期171-175,共5页
Journal of Food Safety and Quality
关键词
二氯二甲吡啶酚
气相色谱-质谱法
鸡肉
鸡肝
检测
dichloro dimethyl pyridine phenol
gas chromatography-mass spectrometry
chicken tissues
chicken liver
determination
