摘要
目的：丰富奶粉中胆碱含量测定的方法,同时对其他同类产品中胆碱含量的测定提供参考。方法样品经盐酸水解,水解液五倍稀释后采用离子色谱仪进行测定。结果离子色谱法对胆碱含量测定的最低检测限为2 mg/kg,相关系数达到0.9998,添加回收率为88.7%~102.5%,平行样标准偏差为0.72~4.15 mg/100 g,相对标准偏差为0.66%~3.30%。结论该方法操作简单,灵敏度高,选择性强,能克服传统方法中繁琐、准确度低等缺陷和不足。
Objective To establish a method for the determination of choline in milk powder and to pro-vide a reference for determination of choline in other similar products. Methods Samples were hydrolyzed using hydrochloric acid, diluted five times using ultrapure water, and determined by ion chromatography. Results The lowest detection limit was 2 mg/kg with correlation coefficient achieve of 0.9998. Recoveries was 88.7%~102.5%, and the standard deviation of parallel samples was 0.72~4.15 mg/100 g with RSD of 0.66%~3.30%. Conclusion This method is simple, sensitive and selective.
出处
《食品安全质量检测学报》
CAS
2013年第6期1880-1884,共5页
Journal of Food Safety and Quality
关键词
离子色谱法法
奶粉
胆碱
chromatography of ions
milk powder
choline
