摘要
目的：建立禽畜肉中利巴韦林的高效液相色谱-串联质谱(HPLC-MS/MS)检测方法。方法待测样品中的利巴韦林用0.25 mol/L醋酸铵缓冲溶液(pH 4.8)提取,经固相萃取柱净化、浓缩、定容后采用液相色谱-串联质谱法在正离子模式下检测。结果该方法在10~300μg/kg范围内线性关系良好,相关系数r>0.999。利巴韦林在禽肉及畜肉中的检测限均为10μg/kg,回收率在97.0%~109.4%之间,精密度(以相对标准偏差表示)在0.7%~2.8%之间(n=6)。结论本方法具有前处理简单、选择性好、灵敏度高等优点,可用于禽畜肉中利巴韦林残留量的定量检测。
Objectives To establish a method for the determination of ribavirin in tissues of livestock and poultry by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Methods The residues of ribavirin in the samples were extracted with buffer (ammonium acetate pH 4.8). After being cleaned up with SPE column, concentrated and reconstituted, the residues were detected by HPLC-MS/MS un-der multiple reaction monitoring (MRM) via positive ionisation mode. Results The limit of quantification was 10μg/kg. The method showed a good linearity over the range of 10~300μg/kg for ribavirin with r>0.999. The average recoveries at 3 spiked concentration levels were between 97.0%~109.4%, while the relative stan-dard deviations (RSD) were between 0.7%~2.8%(n=6). Conclusion The established method is highly sensi-tive, accurate and reproducible. It is suitable for the detection of ribavirin in tissues of livestock and poultry.
出处
《食品安全质量检测学报》
CAS
2014年第3期905-911,共7页
Journal of Food Safety and Quality
关键词
利巴韦林
液相色谱-串联质谱法
固相萃取
禽畜肉
ribavirin
liquid chromatography-tandem mass spectrometry
solid phase extraction
tissues of livestock and poultry
