摘要
核酸适配体是通过指数富集配体系统进化技术经体外筛选得到的可以特异性地识别目标物的一段短的单链寡核苷酸序列。与抗体相比,适配体具有性质稳定、易化学合成、易化学修饰、分子量小和目标分子广泛等优点,目前在生物传感器、毛细管电泳、物质分离富集以及医疗诊断等领域得到了广泛应用。本文重点介绍了核酸适配体在固相萃取、磁分离、亲和色谱及微流控分离分析等样品前处理技术中的应用,并对其应用现状和发展前景进行了综述。
Aptamers are obtained by systematic evolution of ligands by exponential enrichment from a random oligonucleotide library, which exhibit high affinities and specificities to their corresponding targets. Compared with antibody, aptamers are highly stable, easier to synthesize and modify, smaller in size and spe-cific to a wide variety of targets. So far, it has a wide application in the fields of biosensors, capillary electro-phoresis, separation and analysis, medical diagnosis. This article mainly summarizes the applications of apta-mers in sample pretreatment technologies such as solid phase extraction, magnetic separation, affinity chroma-tography, microfluidic formats. What’s more, this review describes the current status and predicates the possible development of aptamers in sample preparations technologies.
出处
《食品安全质量检测学报》
CAS
2014年第5期1305-1313,共9页
Journal of Food Safety and Quality
关键词
核酸适配体
样品前处理
固相萃取
磁分离
亲和色谱
微流控分离
aptamers
sample preparation techniques
solid-phase extraction
magnetic separation
affinity chromatography
microfluidic separation
