摘要
根据减蛋综合征病毒PV 蛋白基因序列设计了1对特异性引物,应用降落PCR法对PV 蛋白基因进行了扩增。将扩增产物克隆至pMD18-T载体中,经酶切和PCR鉴定,初步证明了目的片段的正确性。进一步核苷酸序列测定表明,所扩增的基因片段长度为0.756kb,共编码250个氨基酸。
PVⅢ protein gene of egg drop syndrome virus (EDSV) was amplified by touchdown PCR (TD-PCR) and was cloned into pMD18-T vector.The recombinant plasmids were identified by digestion with restriction endonuclease and PCR.The sequence analysis showed that the nucleotide sequence of PVⅢ gene was 0.756 kb and encoded with a protein of 250 amino acids.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2004年第7期25-28,共4页
Journal of Northwest A&F University(Natural Science Edition)
基金
河南省自然科学基金项目(0214040027)
