摘要
利用原位杂交细胞化学的方法对纳米磷灰石作用 4 h后的人肝癌细胞进行端粒酶基因的检测来研究纳米磷灰石对人肝癌细胞端粒酶基因的影响 ,探讨纳米磷灰石抗癌的机制。结果显示人肝癌 Bel- 74 0 2组的端粒酶阳性细胞率为 88.4 9% ,顺铂组的端粒酶活性为 2 5 .6 % ,纳米磷灰石组为 6 3.6 % ,二者与 Bel- 74 0 2组端粒酶活性相比呈明显下降 ,并存在明显的差异性 (p<0 .0 1)。
Using the hybridization in situ method to detect the expression of the telomerase gene of human hepatocarcinoma treated with the nano-apatite for 4 h at 37 ℃.To investigate the effect of nano-apatite on the expression of the telomerase gene of human hepatocarcinoma cells and further explore the mechanism of the nano-apatite inhibiting cancer. The hybridization in situ showed that the cytoplasm of the positive cells was stained in yellow or nigger-brown. The positive cell rate of the control group was 88.49%、 the cisplatin group was 25.6% and the nano-apatite group was 63.6%. The activity of telomerase gene was both obviously declined comparing with the control group and the difference had significance (p<0.05,p<0.01). The nano-apatite obviously inhabits the expression of the telomerase gene of human hepatocarcinoma cells.
出处
《武汉理工大学学报》
CAS
CSCD
2004年第7期46-48,共3页
Journal of Wuhan University of Technology
基金
"十五"湖北省科技攻关计划重大项目 (2 0 0 2 AA10 5 A)
关键词
纳米磷灰石
抗肿瘤
端粒酶基因
nano-apatite
hepatocarcinoma cells
telomerase gene
