摘要
以小麦(Triticum aestivum L.)为试验材料,采用完全营养液进行培养,外加适当浓度的过氧化氢(H2O2),同时以不加H2O2为对照,再以不同浓度Pb溶液培养进行Pb胁迫,检测小麦幼苗过氧化物酶(POD)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)的活性及丙二醛(MDA)含量、根系活力及微核率等指标的变化,以探究外源H2O2对Pb胁迫下小麦抗氧化酶及其细胞活性的影响。结果表明,Pb胁迫下小麦幼苗的生长代谢受到一定程度的抑制,主要表现为根系活力下降,POD、SOD、CAT活性增强,MDA含量和微核率上升;经适当浓度H2O2处理后,Pb胁迫小麦幼苗中POD、SOD、CAT活性及根系活力与对照相比有不同程度地上升,MDA含量及微核率下降,这表明外加H2O2对Pb胁迫小麦所受的伤害有一定的缓解作用,可增强小麦幼苗对重金属Pb的抗性。
To explore the effect of exogenous H2O2 on antioxidant enzymes and other physiological indexes of wheat seedlings under lead stress,wheat seedlings cultivated in solution with different concentration of H2O2 were used to observe the variation of activity of peroxidase(POD),superoxide dismutase(SOD),catalase(CAT) and content of malondialdehyde(MDA),root activity and micronucleus rate.The results showed that wheat seedlings were chronically harmed under heavy metal stress,as vigor of wheat seedling root decreased;POD,SOD,CAT activity and MDA content,micronucleus rate increased.After being treated by H2O2 with appropriate concentration,POD,SOD,CAT activity,root vigor increased while MDA content and micronucleus rate decreased in wheat seedlings under lead stress compared to control,indicating that exogenous H2O2 could reduce the damage caused by lead stress.
出处
《湖北农业科学》
北大核心
2013年第5期1007-1011,共5页
Hubei Agricultural Sciences
基金
江苏省教育厅高校自然科学研究项目(05KJD180191)
