摘要
对罗汉果(Siraitia grosvenorii)组培苗采用热处理和剥取茎尖技术进行二次脱毒处理,脱毒效果采用间接酶联免疫法和电镜观察法进行检测。结果表明,适合材料热处理的培养基为MS+0.3 mg/L 6-BA+NAA 0.1 mg/L+0.1 mg/L IBA,热处理材料的顶芽呈锥形,利于剥取茎尖;茎尖分化最佳培养基为MS+0.3 mg/L 6-BA+0.05 mg/L NAA+0.1 mg/L GA3;38.5℃热处理10 d剥取长1.0～1.5 mm的茎尖进行二次脱毒培养,存活率为80.00%,脱毒率为100.00%。
The tissue culture seedlings of Siraitia grosvenorii were treated by heat treatment and tip-stripping to obtain virus-free seedling.ELISA(enzyme-linked-immunosorbent serologic assay) method and electron microscope method were applied for virus detection.The results showed that the suitable medium for heat treatment was MS+0.3 mg/L 6-BA+0.1 mg/L NAA+0.1 mg/L IBA.Terminal bud after heat treatment was conical and easy for stripping stem tip.The optimum medium for inducement of tips was MS+0.3 mg/L 6-BA+0.05 mg/L NAA+0.1 mg/L GA3.Heat treatment for 10 d at 38.5 ℃ twice,stripping shoot-tip for 1.0～1.5 mm long,the survival rate was 80.00%;and the detoxication rate was 100.00%.
出处
《湖北农业科学》
北大核心
2013年第5期1185-1187,共3页
Hubei Agricultural Sciences
基金
广西科技攻关项目(桂科攻0630002-3F)
