摘要
目的探讨p53β在肾癌中的表达与肾细胞癌的病理类型、分期以及核分级的相关性。方法采用逆转录PCR对47例肾细胞癌标本和癌旁正常肾组织进行了检测,并结合肾癌的分级和临床分期进行统计学分析。结果 p53β在肾癌组织中的表达显著高于正常肾组织,其表达与其临床分期有关与病理类型、核分级无关。结论 p53β的表达与肾细胞癌的发生发展有密切关系。p53β可以进一步研究作为肾细胞癌新的诊断分子标记和基因治疗的新的靶点。
Objective To analyze the expression patterns of p53βin RCC at the mRNA levels and their associations with clinical and pathologic factors,and to explore the mechanism of p53B activity in RCC.Methods mRNA expression levels of p53 and p53βwere detected using RT - PCR followed by nested PCR.The data were also analyzed with clinicopathological features by x^2 test or Fisher's exact test.Results p53 mRNA was expressed in all tumors and matched clinically normal tissue adjacent to the tumour.p53βmRNA was significantly overexpressed in tumour samples(P <0.001),and correlated with tumour stage.Conclusions p53βmight play an important role in the tumor formation in RCC and might be used as a new marlcer and target for RCC.
出处
《泌尿外科杂志（电子版）》
2010年第1期17-20,共4页
Journal of Urology for Clinicians(Electronic Version)
