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Pentoxifylline improves liver regeneration through downregulation of TNF-α synthesis and TGF-β1 gene expression 被引量：2

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摘要 AIM:To investigate the mechanism of pentoxifylline(PTX)improvement in liver regeneration.RESULTS:Rats were randomized into 4 groups:Control rats;Sham-sham-operation rats;Saline-70% hepatectomy plus saline solution;PTX-70%hepatectomy plus PTX.At 2 and 6 h after hepatectomy,aspartate aminotransferase,alanine aminotransferase,tumor necrosis factor(TNF)-α and interleukin-6(IL-6)serum and hepatic tissue levels were determined.Tumor growth factor(TGF)-β1 gene expression in liver tissue was evaluated 24 h after hepatectomy by quantitative reverse transcriptase polymerase chain reaction analysis.Proliferation was analyzed by mitotic index and proliferating cell nuclear antigen(PCNA)staining 48 h after hepatectomy.RESULTS:TNF-α and IL-6 serum levels increased at 2 and 6 h after hepatectomy.At 2 h after hepatectomy serum PTX was reduced but not hepatic levels of TNF-α and IL-6.A decrease in liver TGF-β1 gene expression and an increase in mitotic index and PCNA after hepatectomy were observed in the PTX treatment group in comparison to the saline group.CONCLUSION:PTX improves liver regeneration by a mechanism related to down regulation of TNF-α production and TGF-β1 gene expression. AIM: To investigate the mechanism of pentoxifylline (PTX) improvement in liver regeneration. RESULTS: Rats were randomized into 4 groups: Control rats; Sham - sham-operation rats; Saline - 70% hepatectomy plus saline solution; PTX - 70% hepatectomy plus PTX. At 2 and 6 h after hepatectomy, aspartate aminotransferase, alanine aminotransferase, tumor necrosis factor (TNF)-α and interleukin-6 (IL-6) serum and hepatic tissue levels were determined. Tumor growth factor (TGF)-β1 gene expression in liver tissue was evaluated 24 h after hepatectomy by quantitative reverse transcriptase polymerase chain reaction analysis. Proliferation was analyzed by mitotic index and proliferating cell nuclear antigen (PCNA) staining 48 h after hepatectomy. RESULTS: TNF-α and IL-6 serum levels increased at 2 and 6 h after hepatectomy. At 2 h after hepatectomy serum PTX was reduced but not hepatic levels of TNF-α and IL-6. A decrease in liver TGF-β1 gene expression and an increase in mitotic index and PCNA after hepatectomy were observed in the PTX treatment group in comparison to the saline group. CONCLUSION: PTX improves liver regeneration by a mechanism related to down regulation of TNF-α production and TGF-β1 gene expression.
作者 Rodrigo Bronze Martino Ana Maria Mendona Coelho Márcia Saldanha Kubrusly Regina Leito Sandra Nassa Sampietre Marcel Cerqueira Cesar Machado Telesforo Bacchella Luiz Augusto Carneiro D’Albuquerque
机构地区 Department of Gastroenterology(LIM/
出处 《World Journal of Gastrointestinal Surgery》 SCIE CAS 2012年第6期146-151,共6页 世界胃肠外科杂志（英文版）（电子版）
关键词 Liver regeneration HEPATECTOMY PENTOXIFYLLINE TUMOR NECROSIS FACTOR α TUMOR growth FACTOR β1 Liver regeneration Hepatectomy Pentoxifylline Tumor necrosis factor α Tumor growth factor β1
分类号 R657.3 [医药卫生—外科学]
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参考文献34

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二级参考文献11

  • 1Wen-Jun Yang,Qi-Yu Zhang,Zheng-Ping Yu,Qi-Tong Song,Hua-Ping Liang,Xiang Xu,Guan-Bao Zhu,Fei-Zhao Jiang,Hong-Qi Shi.Effects of nuclear factor-kappaB on rat hepatocyte regeneration and apoptosis after 70% portal branch ligation[J].World Journal of Gastroenterology,2005,11(43):6775-6779. 被引量：7
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共引文献11

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二级引证文献8

World Journal of Gastrointestinal Surgery
2012年 第6期

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