期刊文献+

Genetic polymorphism and mRNA levels of cytochrome P450ⅡE1 and glutathione S-transferase P1 in patients with alcoholic liver disease in different nationalities 被引量:4

Genetic polymorphism and mRNA levels of cytochrome P450ⅡE1 and glutathione S-transferase P1 in patients with alcoholic liver disease in different nationalities
下载PDF
导出
摘要 BACKGROUND:Alcohol abuse and dependence are major factors in the pathogenesis of alcoholic liver disease(ALD).Alcohol abuse is becoming an increasingly severe problem among the Han,Mongol,and Korean nationalities in northeast China.This study aimed to investigate the relationship between ALD and the genetic polymorphism and expression levels of two enzymes,cytochrome P450ⅡE1(CYPⅡE1)and glutathione S-transferase P1(GSTP1)in patients of three nationalities.METHODS:Peripheral blood was collected from 353 Chinese patients with ALD,300 alcohol dependent patients without liver disease(alcoholic),and 360 healthy controls.Each group included patients from the Han,Mongol and Korean nationalities.Real-time polymerase chain reaction(PCR)and PCR-restriction fragment length polymorphism(PCR-RFLP)were used.RESULTS:Regardless of nationality,patients who carried the rare CYPⅡE1 C2 and GSTP1 Val alleles were at higher risk of ALD.The frequency of C2 and Val in patients with ALD was respectively 50.00%and 26.98%in the Han,31.36%and 22.87%in the Mongol,and 45.87%and 22.02% in the Korean nationality.No significant differences were seen in the frequency of either C2 or Val alleles in ALD patients among the three nationalities.In each nationality,the frequency of both C2 and Val alleles was significantly higher in ALD compared to alcoholic and healthy controls.Except for nationality,the average mRNA levels of CYPⅡ E1 in ALD patients and healthy controls were 10.05%and 2.21%,respectively.The average mRNA levels of GSTP1 in ALD patients and healthy controls were 0.53%and 2.12%,respectively.The mRNA level of CYPⅡE1 was higher,and that of GSTP1 was lower in patients with ALD compared to the controls.CONCLUSIONS:Except for nationality,patients with ALD in this series tended to have a higher mRNA expression of CYPⅡE1 and to carry the C2 allele,and tended to have a lower mRNA expression of GSTP1 and to carry the Val allele.There is a causal relationship between the polymorphic alleles,which leads to different mRNA levels and the development of ALD. BACKGROUND:Alcohol abuse and dependence are major factors in the pathogenesis of alcoholic liver disease(ALD).Alcohol abuse is becoming an increasingly severe problem among the Han,Mongol,and Korean nationalities in northeast China.This study aimed to investigate the relationship between ALD and the genetic polymorphism and expression levels of two enzymes,cytochrome P450ⅡE1(CYPⅡE1)and glutathione S-transferase P1(GSTP1)in patients of three nationalities.METHODS:Peripheral blood was collected from 353 Chinese patients with ALD,300 alcohol dependent patients without liver disease(alcoholic),and 360 healthy controls.Each group included patients from the Han,Mongol and Korean nationalities.Real-time polymerase chain reaction(PCR)and PCR-restriction fragment length polymorphism(PCR-RFLP)were used.RESULTS:Regardless of nationality,patients who carried the rare CYPⅡE1 C2 and GSTP1 Val alleles were at higher risk of ALD.The frequency of C2 and Val in patients with ALD was respectively 50.00%and 26.98%in the Han,31.36%and 22.87%in the Mongol,and 45.87%and 22.02% in the Korean nationality.No significant differences were seen in the frequency of either C2 or Val alleles in ALD patients among the three nationalities.In each nationality,the frequency of both C2 and Val alleles was significantly higher in ALD compared to alcoholic and healthy controls.Except for nationality,the average mRNA levels of CYPⅡ E1 in ALD patients and healthy controls were 10.05%and 2.21%,respectively.The average mRNA levels of GSTP1 in ALD patients and healthy controls were 0.53%and 2.12%,respectively.The mRNA level of CYPⅡE1 was higher,and that of GSTP1 was lower in patients with ALD compared to the controls.CONCLUSIONS:Except for nationality,patients with ALD in this series tended to have a higher mRNA expression of CYPⅡE1 and to carry the C2 allele,and tended to have a lower mRNA expression of GSTP1 and to carry the Val allele.There is a causal relationship between the polymorphic alleles,which leads to different mRNA levels and the development of ALD.
出处 《Hepatobiliary & Pancreatic Diseases International》 SCIE CAS 2009年第2期162-167,共6页 国际肝胆胰疾病杂志(英文版)
基金 supported by a grant from the Youth Science Fund of Heilongjiang Province(No.QC08C77)
关键词 alcoholic liver disease ALCOHOLIC genetic polymorphism mRNA expression alcoholic liver disease alcoholic genetic polymorphism mRNA expression
  • 相关文献

参考文献32

  • 1Veronica Wendy Setiawan,Zuo-Feng Zhang,Guo-Pei Yu,Qing-Yi Lu,Yong-Liang Li,Ming-Lan Lu,Ming-Rong Wang,Chun Hua Guo,Shun-Zhang Yu,Robert C Kurtz,Chung-Cheng Hsieh.GSTP1 polymorphisms and gastric cancer in a high-risk Chinese population[J]. Cancer Causes and Control . 2001 (8)
  • 2Standop J,,Ulrich AB,Schneider MB,Büchler MW,Pour PM.Differences in the expression of xenobiotic- metabolizing enzymes between islets derived from the ventral and dorsal anlage of the pancreas. Pancreatology . 2002
  • 3Wacke R,Kirchner A,Prall F,Nizze H,Schmidt W,Fischer U,et al.Up-regulation of cytochrome P450 1A2,2C9,and2E1 in chronic pancreatitis. Pancreas . 1998
  • 4Lewis D F,Loannides C,Parke D V.Cytochromes P450 and species differences in xenobiotic metabolism and activation of carcinogen. Journal of Environmental Health . 1998
  • 5Boyer TD.The glutathione S-transferases: an update. Hepatology . 1989
  • 6Nebert DW.Role of genetics and drug metabolism in human cancer risk. Mutation Research . 1991
  • 7Guengerich FP,Shimada T.Oxidation of toxic and carcinogenic chemicals by human cytochrome P-450 enzymes. Chemical Research in Toxicology . 1991
  • 8Umeno M,McBride OW,Yang CS,et al.Human ethanol-inducible P450IIE1: complete gene sequence, promoter characterization, chromo some mapping, and cDNA-directed expression. Biochemistry . 1988
  • 9Stephens EA,Taylor JA,Kaplan N,et al.Ethnic variation in the CYP2E1 gene: polymorphism analysis of 695 African-Americans, European-Americans, and Taiwan Residents. Pharmacogenetics . 1994
  • 10Board PG,Webb GC,Coggan M,et al.Isolation of a cDNA clone and localization of the human glutathione S-transferase 3 genes to chromosome bands 11q13 and 12q13-14. Annals of Human Genetics . 1989

共引文献1

同被引文献17

引证文献4

二级引证文献36

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部