摘要
Objective: To demonstrate the utility of DNA vaccinesfor the tailored methods, the efficacy of enhancedimmune responses, and the types of increased im-mune responses.Methods: Four recombinant plasmids constructed in-cluded the coding regions for the core protein （pC）and for the core, E<sub>1</sub> and E<sub>2</sub> together （pCE<sub>1</sub>E<sub>2</sub>）, IL-12 p35 and p40. These plasmids were transfected intomammalian cells to test their protein expression andwere injected into the quadriceps muscles of BALB/C mice for measurement of specific antibodies andcytotoxic T-lymphocyte （CTL） responses.Results: All the recombinant plasmids were shown toexpress specific antigens stably in mammalian cells.Codelivery of pIL-12 expression cassettes with pCand pCE<sub>1</sub>E<sub>2</sub> in mice resulted in the enhancement ofAg-dependent CTL responses and the reduction ofspecific Ab response. The CTL activity was: pC=18.65%±5.71%, pCE<sub>1</sub>E<sub>2</sub>=20.07%±11.11%, pC+pIL-12=60.11%±17.37%, pCE<sub>1</sub>E<sub>2</sub>+pIL-12=67.48%±15.57%, respectively. The average A val-ues of anti-HCV were pC=0.415±0.127, pCE<sub>1</sub>E<sub>2</sub>=0.358±0.096, pC+pIL-12=0.210±0.086, pCE<sub>1</sub>E<sub>2</sub>+pIL-12=0.258±0.125.Conclusion: Codelivery of pIL-12 with plasmid DNAcan enhance the efficacy of immune responses andshift the type of immune responses.
Objective: To demonstrate the utility of DNA vaccines for the tailored methods, the efficacy of enhanced immune responses, and the types of increased im- mune responses. Methods: Four recombinant plasmids constructed in- cluded the coding regions for the core protein (pC) and for the core, E_1 and E_2 together (pCE_1E_2), IL- 12 p35 and p40. These plasmids were transfected into mammalian cells to test their protein expression and were injected into the quadriceps muscles of BALB/ C mice for measurement of specific antibodies and cytotoxic T-lymphocyte (CTL) responses. Results: All the recombinant plasmids were shown to express specific antigens stably in mammalian cells. Codelivery of pIL-12 expression cassettes with pC and pCE_1E_2 in mice resulted in the enhancement of Ag-dependent CTL responses and the reduction of specific Ab response. The CTL activity was: pC= 18.65%±5.71%, pCE_1E_2=20.07%±11.11%, pC +pIL-12=60.11%±17.37%, pCE_1E_2+pIL-12= 67.48%±15.57%, respectively. The average A val- ues of anti-HCV were pC=0.415±0.127, pCE_1E_2= 0.358±0.096, pC+pIL-12=0.210±0.086, pCE_1E_2 +pIL-12=0.258±0.125. Conclusion: Codelivery of pIL-12 with plasmid DNA can enhance the efficacy of immune responses and shift the type of immune responses.
