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Antioxidant activity of newly discovered lineage of marine actinobacteria 被引量:3

Antioxidant activity of newly discovered lineage of marine actinobacteria
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摘要 Objective:To investigate the antioxidant activity of marine actinobacteria.Methods:The content of total phenolics,the level of antioxidant potential by DPPH radical scavenging activity,metal chelating activity,FRAP method,βcarotene assay and NO scavenging activity in extract were determined.Results:In all the methods the extract exhibited good scavenging activity except NO scavenging activity.The IC<sub>50</sub> values of marine actinobacteria extract on DPPH radical were found to be 41.09μg/mL.The zone of color retention was 12 mm inβ-carotene bleaching assay. DNA protective efficiency of the extracts was also studied using UV- photolysed H<sub>2</sub>O<sub>2</sub>-driven oxidative damage to pBR322.HPLC analysis identified some of the major phenolic compounds in extracts,which might he responsible for the antioxidant potential and cyto-protection.It showed a 100%cytotoxic effect in brine shrimp lethality assay within 10 mins.The novel actinobacteria was identified as Streptomyces LK-3(JF710608) through I6S rDNA Sequencing.Conclusions:The results obtained suggest that the extracts bear anti-cancer metabolites and could be considered as a potential source for anti-cancer drug development. Objective:To investigate the antioxidant activity of marine actinobacteria.Methods:The content of total phenolics,the level of antioxidant potential by DPPH radical scavenging activity,metal chelating activity,FRAP method,βcarotene assay and NO scavenging activity in extract were determined.Results:In all the methods the extract exhibited good scavenging activity except NO scavenging activity.The IC50 values of marine actinobacteria extract on DPPH radical were found to be 41.09μg/mL.The zone of color retention was 12 mm inβ-carotene bleaching assay. DNA protective efficiency of the extracts was also studied using UV- photolysed H2O2-driven oxidative damage to pBR322.HPLC analysis identified some of the major phenolic compounds in extracts,which might he responsible for the antioxidant potential and cyto-protection.It showed a 100%cytotoxic effect in brine shrimp lethality assay within 10 mins.The novel actinobacteria was identified as Streptomyces LK-3(JF710608) through I6S rDNA Sequencing.Conclusions:The results obtained suggest that the extracts bear anti-cancer metabolites and could be considered as a potential source for anti-cancer drug development.
出处 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2013年第4期325-332,共8页 亚太热带医药杂志(英文版)
关键词 STREPTOMYCES LK-3 Antioxidant DPPH radical SCAVENGING assay Cytotoxicity Streptomyces LK-3 Antioxidant DPPH radical scavenging assay Cytotoxicity
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