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Free radical scavenging and reducing power of Lawsonia inermis L.seeds 被引量:1

Free radical scavenging and reducing power of Lawsonia inermis L.seeds
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摘要 Objective:To determine the antioxidant activity,total phenolic and flavonoid content of Petroleum ether extract(PE),Dichloromethane extract(DCM),Ethanol extract(ET) and aqueous extract(AQ) of henna seeds.Methods:Total antioxidant assay(phosphomolybenum method), DPPH radical scavenging assay,reducing power assay and lipid peroxidation inhibition assay were used to ascertain the potential of seeds as an antioxidant.Results:In all the assays carried out ET showed a greater potential to scavenge DPPH radical,reduce MO(Ⅳ) to MO(Ⅴ) complex and Fe(Ⅲ) to Fe(Ⅱ) and to inhibit lipid peroxidation.The IC<sub>50</sub> of ET was far greater than that of the standard,ascorbic acid(AS) in the lipid peroxidation assay.The activity of AQ was lesser when compared with that of ET but greater than PE and DCM.The amount of phenolics and flavonoids were present in higher amounts in ET followed by AQ.Trace amounts of phenolics were detected in PE and DCM,but the amount of flavonoids were below the detection level.The study showed that the antioxidant activity and the concentrations of phenolics and flavonoids are proportionate to each other.Conclusions:Ethanolic extract of henna seeds are efficient antioxidants,which can be utilized for further isolation of active compounds and pharmaceutical applications. Objective:To determine the antioxidant activity,total phenolic and flavonoid content of Petroleum ether extract(PE),Dichloromethane extract(DCM),Ethanol extract(ET) and aqueous extract(AQ) of henna seeds.Methods:Total antioxidant assay(phosphomolybenum method), DPPH radical scavenging assay,reducing power assay and lipid peroxidation inhibition assay were used to ascertain the potential of seeds as an antioxidant.Results:In all the assays carried out ET showed a greater potential to scavenge DPPH radical,reduce MO(Ⅳ) to MO(Ⅴ) complex and Fe(Ⅲ) to Fe(Ⅱ) and to inhibit lipid peroxidation.The IC_(50) of ET was far greater than that of the standard,ascorbic acid(AS) in the lipid peroxidation assay.The activity of AQ was lesser when compared with that of ET but greater than PE and DCM.The amount of phenolics and flavonoids were present in higher amounts in ET followed by AQ.Trace amounts of phenolics were detected in PE and DCM,but the amount of flavonoids were below the detection level.The study showed that the antioxidant activity and the concentrations of phenolics and flavonoids are proportionate to each other.Conclusions:Ethanolic extract of henna seeds are efficient antioxidants,which can be utilized for further isolation of active compounds and pharmaceutical applications.
出处 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2011年第6期457-461,共5页 亚太热带医药杂志(英文版)
关键词 Lawsonia inermis ANTIOXIDANT REDUCING free RADICAL Phenolics FLAVONOIDS Lawsonia inermis Antioxidant Reducing free radical Phenolics Flavonoids
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  • 1Chandrasekharan N V,Dai H,Roos K L,et al.COX-3, a cyclooxygenase-1 variant inhibited by acetaminophen and other analgesic/antipyretic drugs: cloning, structure, and expressionProceedings of the National Academy of Sciences of the United States of America,2002.
  • 2Adeolu Adedapo A,Margaret Sofidiya O,viola Maphosa.Anti-inflammatory and analgesic activities of the aqueous extract of Cussonia paniculata stem bark. Rec Nat Prod . 2008
  • 3Bentley GA,Newton SH,Starr J.Studies on the anti-nociceptive action of agonist drugs and their interaction with opioid mechanisms. British Journal of Pharmacology . 1983
  • 4Gene RM,Segura L,Adzet T.Heterotheca inuloides:anti-inflammatory and analgesic effects. Journal of Ethnopharmacology . 1998
  • 5Pasero C,Paice J,McCaffery M.Basic mechanism underlying the causes ands effects of pain. Pain:Clinical manual . 1999
  • 6SW Hajare,C Suresh,SK Tandan.Analgesic and antipyretic activities of Dalbergia sissoo leaves. Indian J. Pharmacol . 2000
  • 7Fhernanda R Smiderle,Lorena M. Olsen,Elaine R. Carbonero,Cristiane H. Baggio.Anti-inflammatory and analgesic properties in a rodentmodel of a (1→3), (1→6)-linked P-glucan isolated from Pleurotus pulmonarius. European Journal of Pharmacology . 2008
  • 8Franzotti E M,,Santos C V,Rodrigues H M,et al.Anti-inflam-matory,analgesic activity and acute toxicity of Sida cardiafoliaL.(Malva-branca). Journal of Ethnopharmacology . 2000
  • 9Bianchi,C,Franceschini,J.Experimental observations on Haffner’s method for testing analgesic drugs. British Journal of Pharmacology . 1954
  • 10Agrahari Ak,Khaliquzzama M,Panda SK.Evaluation of analgesic activity of methanolic extract ofTrapa natans l.var. Bispinosa roxb. Roots. J Current Pharm Res . 2010

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  • 1Habbal O,Hasson SS,El-Hag AH,Al-Mahrooqi Z,Al-Hashmi N,Al-Bimani Z,MS Al-Balushi,Al-Jabri AA.Antibacterial activity of Lawsonia inermis Linn(Henna) against Pseudomonas aeruginosa[J].Asian Pacific Journal of Tropical Biomedicine,2011,1(3):173-176. 被引量:3
  • 2Marjorie Murphy Cowan.Plant products as antimicrobial agents. Clinical Microbiology Reviews . 1999
  • 3Muhammad HS,Muhammad S.The use of Lawsonia inermis linn (henna)in the management of bum wound infections. Afr J Biotechnol . 2005
  • 4Eguale Tadesse,Giday Mirutse.In vitro anthelmintic activity of three medicinal plants against Haemonchus contortus. International Journal of Green Pharmacy . 2009
  • 5Devi K,Thirumaran G,Arumugam R,Anantharuman P.Antibacterial activity of selected medicinal plants from Parangipettai coastal regions;Southeast coast of India. World Appl Sci J . 2009
  • 6A. Janssen,J. Scheffer,A. Svendsen.Antimicrobial Activity of Essential Oils: A 1976-1986 Literature Review. Aspects of the Test Methods[J]. Planta Med . 1987 (05)
  • 7Selma R. de Paiva,Lucilene A. Lima,Maria Raquel Figueiredo,Maria Auxiliadora C. Kaplan.Plumbagin quantification in roots of Plumbago scandens L. obtained by different extraction techniques. An. Acad. Bras. Ciênc . 2004
  • 8Trease GE,Evans WC.Phytochemical screening. Textbook of pharmacognosy . 1989
  • 9Nguyen Xuan Cuong,Nguyen Xuan Nhiem,Nguyen Phuong Thao,Nguyen Hoai Nam,Nguyen Tien Dat,Hoang Le Tuan Anh,Le Mai Huong,Phan Van Kiem,Chau Van Minh,Ji-Hee Won,Won-Yoon Chung,Young Ho Kim.Inhibitors of osteoclastogenesis from Lawsonia inermis leaves[J]. Bioorganic & Medicinal Chemistry Letters . 2010 (16)
  • 10Nighat Sultana,Muhammed Iqbal Choudhary,Ambrin Khan.Protein glycation inhibitory activities of Lawsonia inermis and its active principles[J]. Journal of Enzyme Inhibition and Medicinal Chemistry . 2009 (1)

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