摘要
目的研究瓜蒌桂枝汤对PC12细胞生长的保护作用。方法用脂多糖(LPS)激活小胶质细胞制备炎症细胞模型,用获得的条件培养基对PC12细胞进行干预,用MTT法检测细胞生长活力,用细胞高内涵细胞分析系统对PC12细胞进行形态学观察。结果 LPS激活小胶质细胞后得到的条件培养基能明显抑制PC12细胞生长及形态发生明显改变,瓜蒌桂枝汤干预后,PC12生长活力良好,形态学改变恢复。结论瓜蒌桂枝汤对LPS活化小胶质细胞条件培养基引起的神经细胞损伤具有保护作用。
Objective To investigate the protective effect of Gualou Guizhi decoction(GLGZD) on PC12 cells growth. Methods The model of neuroinflammation was established by stimulating microglia with lipopolysaccharide(LPS), then PC12 cells were treated with condition medium acquired after stimulating microglia with LPS. The viability of PC12 cells was detected by MTT assay, and the morphology was observed by high content analysis system. Results The condition medium acquired after stimulating microglia with LPS could inhibit PC12 cells growth, and the cell morphological changes could be found. After treated with GLGZD, the viability of PC12 cells was improved, and the cells morphology was also ameliorated. Conclusion GLGZD has protective effect on PC12 cells damaged by condition medium acquired after stimulating microglia with LPS.
出处
《福建中医药大学学报》
2014年第3期24-26,共3页
Journal of Fujian College of Traditional Chinese Medicine
基金
福建中医药大学校管课题(X2012010)
