摘要
目的探讨靶向HER2基因shRNA对小鼠肺腺癌Lewis细胞化疗敏感度的影响。方法应用Lipofectamine 2000将pGPU6/RFP/Neo-erbB-2、pGPU6/RFP/Neo-shNC质粒表达载体快速转染小鼠肺腺癌Lewis细胞;应用RT-PCR、Western blot检测各组HER2 mRNA、蛋白的表达。应用流式细胞术检测未转染对照组、pGPU6/RFP/Neo-shNC组、pGPU6/RFP/Neo-erbB-2组、卡铂组、pGPU6/RFP/Neo-shNC+卡铂组、pGPU6/RFP/Neo-erbB-2+卡铂组的细胞凋亡率。结果 pGPU6/RFP/Neo-erbB-2组HER2 mRNA和蛋白的水平均低于pGPU6/RFP/Neo-shNC组和未转染对照组。pGPU6/RFP/Neo-erbB-2+卡铂组细胞的凋亡率高于其余各组(P均<0.01)。结论 HER2 shRNA能增强小鼠Lewis细胞对卡铂的敏感度。
Objective To investigate the effects of HER2 shRNA on the chemotherapy sensitivity of Mice Lewis cells.Methods pGPU6/RFP/Neo-erbB-2 and pGPU6/RFP/Neo-shNC plasmids were synthesized and transfected into mice Lewis cells by Lipofectamine 2000.The levels of HER2 mRNA and protein were tested by RT-PCR and Western blot, respectively.The apoptotic rates of cells in non-transfected group, pGPU6/RFP/ Neo-shNC group,pGPU6/RFP/Neo-erbB-2 group,Carboplatin group, pGPU6/RFP/Neo-shNC plus Carboplatin group and pGPU6/RFP/Neo-erbB-2 plus Carboplatin group were tested by FCM.Results The levels of HER2 mRNA and protein in pGPU6/RFP/Neo-erbB-2 group were lower than those in pGPU6/RFP/Neo-shNC group and non-transfected group.The apoptotic rate of cells in pGPU6/RFP/Neo-erbB-2 plus carboplatin group was higher than those in other groups(all P<0.01).Conclusion HER2 shRNA could increase the sensitivity of mouse Lewis cells to carboplatin.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2014年第5期366-368,共3页
Cancer Research on Prevention and Treatment
基金
四川省卫生厅基金资助项目(100236)
