摘要
目的分析miR-96在肺癌组织中的表达情况;体外研究miR-96反义寡核苷酸(ASO)对肺癌细胞侵袭和迁移的影响。方法运用荧光定量PCR法检测116例肺癌组织及相应癌旁组织中miR-96的表达;通过miR-96 ASO降低肺癌细胞中miR-96的表达,采用Transwell实验和划痕实验观察miR-96ASO对肺癌细胞侵袭和迁移能力的影响,Western blot法检测侵袭相关蛋白的表达变化。结果在116例肺癌病例中,63.80%(74/116)的肺癌组织miR-96表达明显高于相应癌旁组织(P<0.05);与空白对照组和转染随机ASO组相比,miR-96 ASO可以显著降低miR-96的表达(P<0.05);Transwell实验和划痕实验结果显示转染miR-96 ASO后,肺癌细胞的侵袭迁移能力明显下降,同时相关侵袭蛋白MMP2和MMP9表达下降(P<0.05)。结论 miR-96在肺癌组织中表达上调,降低miR-96的表达可有效抑制肺癌细胞的侵袭和迁移。miR-96有望成为肺癌侵袭转移调控的新靶点。
Objective To investigate the expression of miR-96 in lung cancer tissues and demonstrate the regulative effects of miR-96 ASO on the invasion and migration of lung cancer cells in vitro. Methods The expression of miR-96 in 116 cases of lung cancer tissues and their adjacent tissues were detected by fluorogenic quantitative PCR method. The effects of miR-96 ASO on the invasion and migration ability of lung cancer cells were measured by transwell assay and wound healing assay. Invasion-related protein expression was analyzed by Western blot. Results In 116 cases of lung cancer, the expression of miR-96 in 63.80%(74/116) of lung cancer tissues was significantly higher than that in adjacent tissues(P<0.05). miR-96 expression in lung cancer cells in miR-96 ASO transfection group was significantly lower than that in MOCK and NC group(P<0.05). Transwell and wound healing assay results showed that the invasion and migration ability was decreased greatly after transfected with miR-96 ASO, furthermore, down-regulation of miR-96 resulted in obvious inactivation of MMP2 and MMP9(P<0.05). Conclusion miR-96 was up-regulated in human lung cancerous tissue. Reducing the expression of miR-96 can effectively inhibit the invasion and migration of lung cancer cells. miR-96 may become a new target for regulating the invasion and migration ability in lung cancer.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2014年第6期588-592,共5页
Cancer Research on Prevention and Treatment
关键词
miR-96
肺癌
侵袭
迁移
反义寡核苷酸
miR-96
Lung cancer
Invasion
Migration
Antisense oligonucleotides(ASO)
