摘要
Elucidation of maternal-fetal tolerance mechanisms clarifies the role of regulatory T cells (Treg) in transplant tolerance. This study aim to investigate the effect of pregnancy on paternal skin allograft survival. Flow cytometry techniques, mixed lymphocytes reaction (MLR), PCR, real-time PCR and skin transplantation were key methods. Treg increased significantly from 4.2% before pregnancy to peak at 6.8% day 8 after pregnancy. Both heme oxygenase-1 (HO-1) and indoleamine 2,3-dioxygenase (IDO) mRNA express high in placenta while low in spleen (P<0.05). Although Treg increased during pregnancy, and splenocytes from the pregnant mice showed lower MLR response toward the paternal stimulator, single time pregnancy showed no significant protective effect on paternal skin allograft survival in the tested condition.
Elucidation of maternal-fetal tolerance mechanisms clarifies the role of regulatory T cells (Treg) in transplant tolerance. This study aim to investigate the effect of pregnancy on paternal skin allograft survival. Flow cytometry techniques, mixed lymphocytes reaction (MLR), PCR, real-time PCR and skin transplantation were key methods. Treg increased significantly from 4.2% before pregnancy to peak at 6.8% day 8 after pregnancy. Both heme oxygenase-1 (HO-1) and indoleamine 2,3-dioxygenase (IDO) mRNA express high in placenta while low in spleen (P<0.05). Although Treg increased during pregnancy, and splenocytes from the pregnant mice showed lower MLR response toward the paternal stimulator, single time pregnancy showed no significant protective effect on paternal skin allograft survival in the tested condition.
作者
SHOU ZhangFei1,2,3, XU YiFang2, XIAO HuaYing2, ZHOU Qin2, CAI JieRu2, YANG Yi1, JIANG Hong2, ZHANG WenJie3 & CHEN JiangHua1,2 1 Kidney Disease Center, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China
2 Key Laboratory of Multi-organ Combined Transplantation, Ministry of Health, Hangzhou 310003, China
3 Key Laboratory of Xinjiang Endemic and Ethnic Diseases, Ministry of Education, School of Medicine, Shihezi University 832003, China
基金
Supported by the National Natural Science Foundation of China (Grant Nos. 30471641 and 30872389)
the Natural Science Foundation of Zhejiang Province (Grant No. Y207088)
