摘要
目的研究抗老年痴呆软胶囊中五味子醇甲含量的测定方法。方法样品用甲醇回流提取进样,色谱柱:Agilent ZORBAX SB-C18柱(250mm×4.6mm,5μm),流动相:乙腈-水(45∶55),检测波长:250nm,流速:1mL·min-1。结果五味子醇甲在0.046~0.92μg之间线性关系良好,r=0.9999(n=7),平均回收率98.51%,RSD为1.84%(n=6)。结论本法简便,快速,准确,可用于本制剂及五味子药材的含量测定及质量控制。
OBJECTIVE To establish an HPLC method for the determination of Schisandrin in Kanglaonianchidai soft capsule.METHODS HPLC conditions were as follows:Agilent ZORBAX SB-C18(250 mm×4.6 mm,5 μm)column,acetonitrile-H2O(45∶55)as mobile phase with flow rate of 1 mL·min-1,and the detection wavelength at 250 nm.RESULTS The calibration curve was linear between 0.046-0.92 μg(r=0.999 9,n=7).The average recovery was 98.51% and RSD=1.84%(n=6).CONCLUSION The method is convenient and efficient,and can be used for quantitative analysis and quality control of this preparation.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2009年第S1期1146-1148,共3页
Chinese Journal of Modern Applied Pharmacy
基金
浙江省科技计划重点项目(2007C23025)
