摘要
目的建立高效液相色谱法测定复方双花藤止痒搽剂中苦参碱的含量。方法采用色谱柱为Thermo ODS Hypersil(4.6mm×250mm,5μm),以乙睛-磷酸盐缓冲液(取磷酸二氢钾1.36g,加1mL三乙胺,加水至1000mL,用磷酸调pH至5.5)(4∶96)为流动相,检测波长220nm,流速1.0mL·min-1,柱温为35℃。结果苦参碱在0.43~21.44μg内有良好的线性关系(r=0.9999),平均回收率为100.40%,RSD为1.93%(n=6)。结论该方法结果准确可靠,重复性好,可用于复方双花藤止痒搽剂中苦参碱的含量测定。
OBJECTIVE To establish a RP-HPLC method for the determination of matrine in Fufang Shuanghuateng Zhiyang liniment.METHODS The Thermo ODS Hypersil column(250 mm×4.6 mm,5 μm)was used with the acetonitrile-phosphate buffer solution(contained 1.36 g potassium dihydrogen phosphate and 1 mL triethylamine,add water to 1 000 mL adjusted pH to 5.5 with phosphate acid)(4∶96)as mobile phase,the detection wavelength was 220 nm,the flow rate was 1.0 mL·min-1,the temperature of the column was 35 ℃。RESULTS The linear range of matrine was at 0.43-21.44 μg(r=0.999 9,the average recovery was 100.40% with RSD of 1.93%(n=6).CONCLUSION The method is accurate and reliable,it can be used for the determination of matrine in Fufang Shuanghuateng Zhiyang liniment.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2009年第S1期1155-1157,共3页
Chinese Journal of Modern Applied Pharmacy
