摘要
采用“逆相蒸发法”制备含有牛血细胞超氧化物歧化酶(Cu,2n—SOD)的大单层囊泡脂质体,经过一次Sepharose 4B柱层析得到纯化的SOD—脂质体(SOD—Luv);SOD—Luv可以有效地抵抗6.Omol/L盐酸胍的钝化作用;SOD—Luv可以有效地抵抗胰蛋白酶的消化作用。
In this paper,large unilamellar vesicles(LUV)containing SOD was gained by Reverse Evaporation,and it was purified by Sepharose 4B chromatography. The results of de-naturation by GuHcl and of hydrolysis by trypsin showed the SOD-LUV had higher stability than SOD.
出处
《湖北师范学院学报（自然科学版）》
1998年第6期66-68,共3页
Journal of Hubei Normal University(Natural Science)
基金
省教委重点资助项目
