摘要
为了探讨Ⅷ因子相关抗原（Ｆ８ＲＡ／ｖＷＦ）显示肿瘤新生血管的可靠性及方法学。我们对８５例非小细胞肺癌（ｎｏｎｓｍａｌｃｅｌｌｕｎｇｃａｒｃｉｎｏｍａ，ＮＳＣＬＣ）的石蜡组织切片进行Ｆ８ＲＡ／ｖＷＦ免疫组化染色，结果显示：Ｆ８ＲＡ／ｖＷＦ免疫组化清楚地选择性地显示血管内皮细胞，肿瘤细胞和其它正常组织不着色，肿瘤内有高密度微血管区。微血管数目均值为７１±３９，Ⅰ期ＮＳＣＬＣ微血管数目均数为６５２±３４８，腺癌为７６６±３９２。本结果与其他研究结果比较：Ｆ８ＲＡ／ｖＷＦ免疫组化显示肿瘤新生血管有一定的可靠性。
In order to study the reliability and methodology of factor Ⅷ related antigen/von Willebrands factor(F8RA/vWF) staining in the detection of neovascularization of tumor, paraffin tissue sections were stained for F8RA/vWF by a standard immunohistochemistry technique in 85 cases of non small cell lung carcinomas (NSCLC). The results showed that F8RA/vWF positive staining provided reliable discrimiration of endothelial cells. Tumor cells, as well as other normal tissue constituents, were consistently unstained. There were the highest neovascularization areas in intratumor region. The mean microvessel counts, mean microvessel counts in stage Ⅰ and mean micovessel counts in adenocarcinoma were 71±3 9、65 2±34 8 and 76 6±39 2 respectively. Comparing our results with others, it is concluded that the F8RA/vWF staining has been shown to be reliable to evaluate the neovascularization of tumors. A standard immunohistochemistry technique and reproducible method of microvesel measurement are needed.
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
1998年第3期124-127,共4页
Chinese Journal of Histochemistry and Cytochemistry
关键词
Ⅷ因子相关抗原
免疫组化
方法学
非小细胞肺癌
Factor Ⅷ related antigen
\ Immunohistochemistry
\ Methodology
\ Non small cell lung carcinoma
